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Addgene

We narrowed to 7,064 results for: tet on

Showing: 3861 - 3880 of 7064 results
  1. pAraH6HATT

    Plasmid
    #63901
    Purpose
    For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.
    Depositor
    Insert
    TT Fab
    Use
    Tags
    6xHis and HA tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pHis17_ParM _2

    Plasmid
    #78202
    Purpose
    Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor
    Depositor
    Insert
    parM (K33A D63C T174A T175N D224C C287A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    K33A D63C T174A T175N D224C C287A
    Promoter
    unknown
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. MK1274

    Plasmid
    #71431
    Purpose
    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.
    Depositor
    Insert
    Mixed Feedback Loop version of the UBER system
    Use
    Tags
    Expression
    Mutation
    T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…
    Promoter
    Available since
    Dec. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pComb3HTT

    Plasmid
    #63900
    Purpose
    Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.
    Depositor
    Insert
    TT Fab
    Use
    Phage display
    Tags
    geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pDB007ns2a

    Plasmid
    #99214
    Purpose
    Accessory plasmid for PACE of PylRS variants; negative selection
    Depositor
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and Ptet
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pKS014

    Plasmid
    #65467
    Purpose
    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidase
    Depositor
    Insert
    Ntag-beta galactosidase
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    pl-TetO
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  8. pREVTRE-AID

    Plasmid
    #121067
    Purpose
    Retrovirus vector expresses AID-fusion protein under TRE promoter and with hygromycin resistance selectio
    Depositor
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    AID
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pKS011

    Plasmid
    #65464
    Purpose
    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry
    Depositor
    Insert
    MBP-Ntag-mCherry
    Use
    Tags
    FLAG and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    pl-TetO
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. pKS012

    Plasmid
    #65465
    Purpose
    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherry
    Depositor
    Insert
    Ntag-mCherry
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    pl-TetO
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 3861 - 3880 of 7064 results