We narrowed to 11,622 results for: VARS

1. pOmpT

   Plasmid

   #112123

   Purpose

   Bacterial expression outer membrane protein T (OmpT), wild type

   Depositor

   Karen Fleming

   Article

   Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

   Insert

   OmpT (ompT E. coli)
   

   Expression

   Bacterial

   Mutation

   N-terminal signal sequence not included and repla…

   Promoter

   T7

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-mSmo-NQ4-V5

   Plasmid

   #126416

   Purpose

   Localization, Expression of Smoothened NQ4 in mammalian cell lines.

   Depositor

   Stacey Ogden

   Article

   Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

   Insert

   m-Smoothened (Smo Mouse)
   

   Expression

   Mammalian

   Mutation

   Smo glycosylation site NQ4

   Available Since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ2241 - [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)

   Plasmid

   #159860

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_UBASH3A-1/1

   Plasmid

   #91478

   Purpose

   Protein expression and purification of human SH3 domain construct UBASH3A-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   UBASH3A-1/1 (UBASH3A Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221 Cas9-HMGB1

   Plasmid

   #183198

   Purpose

   Entry clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   Cas9-HMGB1
   

   Use

   CRISPR; Gateway entry clone

   Mutation

   n/a

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. GTU-g

   Plasmid

   #81094

   Purpose

   Gene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   sfGFP

   Expression

   Yeast

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOmpW

   Plasmid

   #112121

   Purpose

   Bacterial expression of outer membrane protein W (OmpW), wild type

   Depositor

   Karen Fleming

   Article

   Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

   Insert

   OmpW (ompW E. coli)
   

   Expression

   Bacterial

   Mutation

   N-terminal signal sequence not included and repla…

   Promoter

   T7

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

   Plasmid

   #159801

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221 Cas9-RFC4

   Plasmid

   #183199

   Purpose

   Entry clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   Cas9-RFC4
   

   Use

   CRISPR; Gateway entry clone

   Mutation

   n/a

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG HDAC5 S498A

    Plasmid

    #32215

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser498Ala

    Promoter

    CMV

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-GFAP104-ChromeQ-mCherry

    Plasmid

    #123125

    Purpose

    AAV-mediated expression of ChromeQ-mCherry under the GFAP promoter.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeQ-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

    Promoter

    GFAP104

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_NCK1-3/3

    Plasmid

    #91476

    Purpose

    Protein expression and purification of human SH3 domain construct NCK1-3/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCK1-3/3 (NCK1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTFP1-Tubulin-6

    Plasmid

    #55512

    Purpose

    Localization: Microtubules, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pHH0103_SASH1-1/1

    Plasmid

    #91347

    Purpose

    Protein expression and purification of human SH3 domain construct SASH1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SASH1-1/1 (SASH1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pProvector_AAV2_586_PLGLAR

    Plasmid

    #129024

    Purpose

    Recombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.

    Depositor

    Junghae Suh

    Article

    Tong et al J Control Release. 2019 Jun 25. pii: S01

    Insert

    Rep2/Cap2 with PLGLAR lock at 586
    

    Use

    AAV

    Mutation

    inserted peptide motif (PLGLARGDDDDGPLGLAR) at po…

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

    Plasmid

    #159864

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CRISPR sgSHMT1_2

    Plasmid

    #106312

    Purpose

    Express Cas9 and sgRNA targeting SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_SLA2-1/1

    Plasmid

    #91450

    Purpose

    Protein expression and purification of human SH3 domain construct SLA2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SLA2-1/1 (SLA2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_NCK2-2/3

    Plasmid

    #91362

    Purpose

    Protein expression and purification of human SH3 domain construct NCK2-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCK2-2/3 (NCK2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMS7: pCascade(AvCAST)_entry

    Plasmid

    #168140

    Purpose

    Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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