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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,186 results for: egf

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Showing: 3881 - 3900 of 15186 results

  2. pLenti-PGK-EGFP-ALDH1A2-Neo

    Plasmid
    #189743
    Purpose
    The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.
    Depositor
    Robert Sobol
    Publication
    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ALDH1A2 (ALDH1A2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    189743-plasmid-map-sequence-id-374852

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  5. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    160140-plasmid-map-sequence-id-317331

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  7. pCS6-ZF(VEGFA)-StaPL(AI)-YFP-VPR

    Plasmid
    #111502
    Purpose
    Expresses a zinc finger specific to the human VEGFA locus, which is linked to a VPR transcriptional activation domain via a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].
    Depositor
    Michael Lin
    Publication
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    ZF(VEGFA)-StaPL(AI)-YFP-VPR
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries V36M, T54A, and S122…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111502-plasmid-map-sequence-id-214269

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  10. pcDNA-ELYS-emerin-EGFP-polyA

    Plasmid
    #59745
    Purpose
    Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
    Depositor
    Yi Zhang
    Publication
    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ELYS-emerin-EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59745-plasmid-map-sequence-id-93966

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  15. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

    Plasmid
    #122964
    Purpose
    Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Insert
    human Chibby1 (CBY1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Promoter
    FoxJ1 and human FoxJ1
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122964-plasmid-map-sequence-id-291837

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  16. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid
    #140000
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    140000-plasmid-map-sequence-id-275479

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  18. pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

    Plasmid
    #208696
    Purpose
    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells
    Depositor
    Yulong Li
    Publication
    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    208696-plasmid-map-sequence-id-412358

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  19. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid
    #185916
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185916-plasmid-map-sequence-id-367354

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Showing: 3881 - 3900 of 15186 results