We narrowed to 12,189 results for: LEA
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Plasmid#137817Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v6 (-cyt) (CD44 Human)
TagsGFPExpressionMammalianMutationwithout cytoplasmic region, R296K (please see dep…PromoterPGKAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 LNG-6MT
Plasmid#41738DepositorInsertNotch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
Tags6xMycExpressionMammalianMutationContains murine Notch-1 leader peptide (aa 1-23) …Available sinceJan. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecB GamS
Bacterial strain#176585PurposeE. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.DepositorBacterial resistanceChloramphenicol and AmpicillinAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV Retrograde)
Viral prep#125712-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromotershort version of αCamKinase II promoter (0.4kb)Available sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)
Plasmid#223125PurposepCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
UseCRISPRTags2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDdExpressionMammalianMutationTadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)
Plasmid#208959PurposeA variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPSint
Plasmid#149421PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene.DepositorTypeEmpty backboneExpressionPlantAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPSup2
Plasmid#149418PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted about 1.4 kb upstream of the minimal promoter.DepositorTypeEmpty backboneExpressionPlantAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSdown
Plasmid#149417PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the 3'-UTR of the GFP reporter gene.DepositorTypeEmpty backboneExpressionPlantAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIC
Plasmid#27989DepositorTypeEmpty backboneExpressionBacterialAvailable sinceMarch 22, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmCer-Treslin
Plasmid#211404Purposemammalian expression of human Treslin N-terminally fused to mCeruleanDepositorAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z03
Plasmid#120046PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3DepositorInsertmoxCerulean3
UsePcr templateTagsmoxCerulean3PromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS10
Plasmid#83795PurposeUseful for tagging yeast proteins with C-terminal Cerulean tag; hygromycin-B selectionDepositorPublicationInsertCerulean
TagsCeruleanExpressionYeastAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-H2GH
Plasmid#182202PurposeAdeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion constructDepositorInserthistone2B-HaloTag-GFP
UseAAVAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMINTNH3
Plasmid#110074PurposeIntegrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage siteDepositorTypeEmpty backboneTagsHis10 Tag - 3C cleavage siteExpressionBacterialAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Banshee-Sh-Ctrl
Plasmid#85587PurposeControl hairpin sequence used in gene knockdown experiementsDepositorInsertsh-Ctrl
UseRetroviralExpressionMammalianPromoterH1Available sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>
Plasmid#72240PurposeHistidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)DepositorInsertGLT1 GAL1p 24xPP7 V3
ExpressionBacterialAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-U6-sgRNA-CMV-puro
Plasmid#169450Purposebackbone for sgRNA expressionDepositorInsertsgRNA
UseLentiviralAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-STRNDHFR
Plasmid#63978PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTagsStrep II (STR); TEV cleavableExpressionBacterialPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2
Plasmid#221712PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.DepositorAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only