We narrowed to 31,181 results for: LEA

1. pcDNA3-mCherry-TSC2

   Plasmid

   #192440

   Purpose

   Encodes mCherry-tagged wild-type TSC2 isoform 5.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2 (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCL1032

   Plasmid

   #217962

   Purpose

   Mammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.

   Depositor

   Andrew Bowman

   Article

   Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

   Insert

   NASP (NASP Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-NLS-PhoCl-mCherry

   Plasmid

   #87691

   Purpose

   Express NLS-PhoCl-mCherry in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   NLS-PhoCl-mCherry
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. M-NMn-VP64

   Plasmid

   #48676

   Purpose

   Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Expression

   Mutation

   nuclease-null

   Promoter

   CMV

   Available since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. M-ST1n-VP64

   Plasmid

   #48675

   Purpose

   Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Expression

   Mutation

   nuclease-null (D9A D598A H599A N622A)

   Promoter

   CMV

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30b-5M SrtA with TEV site

   Plasmid

   #86962

   Purpose

   Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

   Depositor

   Ron Bose

   Article

   Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

   Insert

   S.aureus SrtA 5M
   

   Use

   Tags

   6x Histidine tag and TEV site

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-H2GH

   Plasmid

   #182202

   Purpose

   Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

   Depositor

   Joshua Kritzer

   Article

   Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

   Insert

   histone2B-HaloTag-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas1_Cas2/3

   Plasmid

   #89240

   Purpose

   Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas1 and Cas2/3
   

   Use

   Tags

   Strep II tag on Cas1

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJFRC7-UAS-NPRR_ANP

   Plasmid

   #133243

   Purpose

   Fluorescent reporter for neuropeptide release imaging

   Depositor

   David Anderson

   Article

   Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

   Insert

   rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMBPCas2/3

    Plasmid

    #89238

    Purpose

    Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

    Depositor

    Blake Wiedenheft

    Article

    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

    Insert

    Cas2/3
    

    Use

    Tags

    Strep II-MBP

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. M-SPn-VP64

    Plasmid

    #48674

    Purpose

    Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null

    Promoter

    CMV

    Available since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pAcGFP-Jpx9C104T

    Plasmid

    #120381

    Purpose

    Reporter for RNA nuclear localization

    Depositor

    Igor Ulitsky

    Article

    Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

    Insert

    JPX (JPX Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    C321T

    Promoter

    CMV

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV HDM2(NES-)

    Plasmid

    #12087

    Depositor

    Tyler Jacks

    Article

    Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

    Insert

    HDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Nuclear-export sequence mutant (NES-): L205A, I20…

    Promoter

    Available since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

14. NKP64 GFP (1007)

    Plasmid

    #62020

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    SLC38A10 (SLC38A10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

15. HisMBP CFlm25

    Plasmid

    #78458

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie

    Article

    Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-

    Insert

    Human Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCLR2070

    Plasmid

    #28063

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains N496D point mutation in FokI domain, as …

    Promoter

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCLR2068

    Plasmid

    #28062

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains H537R point mutation in FokI domain, as …

    Promoter

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUPD2+pCmYLCV911

    Plasmid

    #170878

    Purpose

    Phytobrick - pCmYLCV911

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    pCmYLCV911
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBW-m4046921

    Plasmid

    #145755

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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