We narrowed to 8,728 results for: Set

1. Anti-MBP [N128A/4R]

   Plasmid

   #206531

   Purpose

   Mammalian Expression Plasmid of anti-Maltose binding protein (MBP) (E. coli). Derived from hybridoma N128A/4.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Maltose binding protein (MBP) (Escherichia coli) recombinant Mouse monoclonal antibody (malE Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-VGlut1 [N28/9R-1]

   Plasmid

   #206706

   Purpose

   Mammalian Expression Plasmid of anti-VGlut1 neurotransmitter transporter (Rat). Derived from hybridoma N28/9-1.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-VGlut1 neurotransmitter transporter (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc17a7 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV_ACE2-Flag_Hygro

   Plasmid

   #191998

   Purpose

   Lentiviral vector to generate flag-tagged ACE2 stable expressing cell line

   Depositor

   Jan Carette

   Article

   Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-hSyn-PROGERIN

   Plasmid

   #140189

   Purpose

   Overexpression of Progerin with a GFP fusion protein, under human synapsin promotor

   Depositor

   Lorenz Studer

   Article

   Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

   Insert

   Progerin (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4.23-GW

   Plasmid

   #60323

   Purpose

   This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Anti-6xHis [N144/14-rat IgG2a] - Chimeric

   Plasmid

   #227037

   Purpose

   Mammalian expression plasmid of anti-6xHis rat IgG2a R-mAb. Derived from hybridoma N144/14.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-6xHis recombinant mouse monoclonal antibody.
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

   Plasmid

   #67979

   Purpose

   Cas9 activity reporter (control) with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

   Plasmid

   #67981

   Purpose

   Cas9 activity reporter (control) with mCherry and GFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

   Plasmid

   #67982

   Purpose

   Cas9 activity reporter with mCherry and GFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. GABA(A)R, Delta scFv [N151/3]

    Plasmid

    #206724

    Purpose

    Mammalian Expression of GABA(A)R, Delta scFV. Derived from hybridoma N151/3 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    GABA(A)R, Delta (Rattus norvegicus) recombinant scFV (Gabrd Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

    Plasmid

    #67976

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmAG cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS1096 Dual-sgRNA.Design 1

    Plasmid

    #159538

    Purpose

    Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

    Depositor

    Erik Sontheimer

    Article

    Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

    Insert

    Nme2Cas9 nuclease with two guide RNA cassettes with promoters
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

    Plasmid

    #135814

    Purpose

    Fluorescent reporter for knock-in of TdTomato into the TH locus

    Depositor

    Tim Ahfeldt, Lee Rubin

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
    

    Tags

    p2a-TdTomato

    Expression

    Mammalian

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

    Plasmid

    #67991

    Purpose

    CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

    Plasmid

    #67985

    Purpose

    Cas9 activity reporter (control) with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. VGAT scFv [L118/14]

    Plasmid

    #206754

    Purpose

    Mammalian Expression of VGAT scFV. Derived from hybridoma L118/14 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    VGAT (Mus musculus) recombinant scFV (Slc32a1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. peSCBE3-NG-Hypa

    Plasmid

    #218165

    Purpose

    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutation in the portion of deaminase hAPOBEC3A : …

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Kv1.2 K+ channel scFv [K14/16]

    Plasmid

    #182064

    Purpose

    Mammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
    

    Tags

    HA, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. GAD67 scFv [L127/8]

    Plasmid

    #190509

    Purpose

    Mammalian Expression of GAD67 scFV. Derived from hybridoma L127/8.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    GAD67 (Homo sapiens) recombinant scFV (GAD1 Mouse)
    

    Tags

    Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Kv2.1 K+ channel scFv [K39/25]

    Plasmid

    #182067

    Purpose

    Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K39/25.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)
    

    Tags

    HA, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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