We narrowed to 166,040 results for: addgene

1. hAAVS1 TALEN Left

   Plasmid

   #52341

   Purpose

   TALEN vector for genomic targeting

   Depositor

   Su-Chun Zhang

   Article

   Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

   Insert

   AAVS1 TALEN Left
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. hAAVS1 TALEN Right

   Plasmid

   #52342

   Purpose

   TALEN vector for genomic targeting

   Depositor

   Su-Chun Zhang

   Article

   Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

   Insert

   AAVS1 TALEN Right
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pROSA26-ERT2CreERT2

   Plasmid

   #149436

   Purpose

   ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

   Depositor

   Primo Schaer

   Article

   Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

   Insert

   ERT2-Cre-ERT2 fusion protein
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   ERT2

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. mc2 puro

   Plasmid

   #206215

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ccas9LacZ

   Plasmid

   #113083

   Purpose

   Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe neo TGFBR3-HA

   Plasmid

   #83095

   Purpose

   Retroviral vector for constitutive TGFBR3 expression with C-terminal HA tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   TGFBR3 (TGFBR3 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

7. pAGM4723:TpCC_Urease

   Plasmid

   #85982

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Inserts

   Cas9
   Urease-targeting gRNA 1
   Urease-targeting gRNA 2
   

   Use

   CRISPR; Diatom (t. pseudonana)

   Tags

   NLS and YFP

   Promoter

   FCP and U6

   Available Since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mc2 mCherry

   Plasmid

   #206219

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mc2 neo

   Plasmid

   #206214

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TAL2289

    Plasmid

    #36724

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FANCG-TALEN_Right (Fancg Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-FoxO1-Clover

    Plasmid

    #67759

    Purpose

    Fluorescent fusion protein for tracking Akt signaling

    Depositor

    Peter Rotwein

    Article

    Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

    Insert

    Fox01 (Foxo1 Mouse)
    

    Use

    Lentiviral

    Tags

    Clover

    Mutation

    Changed Serine 209 to Alanine, changed Histidine …

    Promoter

    EF1a

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

    Plasmid

    #191500

    Purpose

    GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

    Depositor

    Michael Granato

    Article

    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

    Insert

    CaSR-EGFP-SV40
    

    Use

    Creation of transgenic fish using tol2 transgenes…

    Promoter

    myo6b

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDECKO_mCherry

    Plasmid

    #78534

    Purpose

    Backbone to clone single or paired sgRNAs

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA)

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MX1_pCSdest

    Plasmid

    #53951

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    MX1 (MX1 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDM068

    Plasmid

    #216811

    Purpose

    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Type

    Empty backbone

    Use

    CRISPR; Fungal expression

    Expression

    Bacterial

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEP163

    Plasmid

    #137881

    Purpose

    PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    mRFP-T2A-BlasticidinR
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFYF1327 EGFP Site#2

    Plasmid

    #47512

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pK.myc-MYPT1

    Plasmid

    #24101

    Depositor

    Masumi Eto

    Article

    Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2

    Insert

    Myosin-targeting subunit-1 (PPP1R12A Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S50G, P415Q

    Available Since

    March 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCASB

    Plasmid

    #190175

    Purpose

    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

    Depositor

    Jessica Tyler

    Article

    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

    Insert

    sgRNA cloning site
    

    Expression

    Bacterial and Yeast

    Mutation

    Added 5'-agagacc-3' upstream and 5'…

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX301-ZIM3-KRAB-PYL1

    Plasmid

    #154761

    Purpose

    Expresses ZIM3 KRAB domain fused to PYL1

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    CMV

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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