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JP705: pMVP/SB/Neo-DEST

Plasmid

#121866

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK001: pMVP/SB/Hygro-DEST

Plasmid

#121867

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JT504: pMVP/SB/eGFP-DEST

Plasmid

#121861

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JT601: pMVP/SB/mCherry-DEST

Plasmid

#121862

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KN302: pMVP/SB/eCFP-DEST

Plasmid

#121863

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK901: pMVP/SB/Zeo-DEST

Plasmid

#121868

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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XRE-pNL1.3[secNLuc]

Plasmid

#182295

Purpose

Secreted Luciferase reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

Xenobiotic Response Element (CYP1A1 Human)

Tags

secNluc

Expression

Mammalian

Promoter

XRE

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2

Plasmid

#75112

Purpose

lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-PDI

Plasmid

#202486

Purpose

Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Human, Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_Luciferase

Plasmid

#25894

Purpose

Gateway entry vector containing Luciferase.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

Luciferase

Use

Gateway entry vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pRPN265NB-enh-MNase

Plasmid

#166035

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

Insert

Glutathione-S-Transferase/enhancer/MNase

Tags

GST

Expression

Bacterial

Promoter

lac promoter / tac promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_LacZ

Plasmid

#25893

Purpose

Gateway entry vector containing LacZ.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

LacZ

Use

Gateway entry vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-DsbC

Plasmid

#202485

Purpose

Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-D3A-3L-dCas9-ZIM3

Plasmid

#240531

Purpose

Expresses MMLVgag–DNMT3A-3L-dCas9-ZIM3 for producing RENDER-DNMT3A-3L-dCas9-ZIM3

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-D3A-3L-dCas9-ZIM3

Tags

FLAG, HA

Expression

Mammalian

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

Plasmid

#216696

Purpose

Synchronize trafficking of TNFalpha from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)

Tags

FIREtag, Streptavidin Binding Protein (SBP), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMY-IRES-eGFP

Plasmid

#163361

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and an IRES separated GFP marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_HcRed

Plasmid

#25892

Purpose

Gateway entry vector containing HcRed.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

hcRed

Use

Gateway entry vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-dCas9-ZIM3

Plasmid

#240527

Purpose

Expresses MMLVgag–dCas9-ZIM3 for producing RENDER-dCas9-ZIM3

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-dCas9-ZIM3

Tags

FLAG, HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pUHD.NFATc1nuc

Plasmid

#40616

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1nuc (NFATC1 Human)

Tags

HA

Expression

Mammalian

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Promoter

CMV2

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-GFPg1 (BB09)

Plasmid

#139461

Purpose

Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

GFP-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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