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GST-HA-GFP11-N1

Plasmid

#91953

Purpose

Expresses GST-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

GST-HA-GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST17-Cdc34a

Plasmid

#18674

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Cdc34A (CDC34 Human)

Tags

His

Expression

Bacterial

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5-AP(-3)-Ub

Plasmid

#232583

Purpose

Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AP

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRho-DsRed

Plasmid

#11156

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

rhodopsin promoter (RHO Bovine)

Tags

DsRed2

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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GW1-ARSeNL

Plasmid

#207873

Purpose

Mammalian expression of the BRET ATP sensor

Depositor

Mathew Tantama

Article

Min et al Sensors (Basel). 2019 Aug 10;19(16). pii

Insert

ARSeNL

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSG86-J23151-LasR

Plasmid

#102456

Purpose

Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

LasR (lasR )

Expression

Bacterial

Promoter

J23151

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP11-His3MX

Plasmid

#91951

Purpose

Universal C-terminal GFP11 tagging

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Type

Empty backbone

Expression

Yeast

Available Since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHR'-NRSF-CITE-GFP

Plasmid

#21310

Depositor

Jay Nadeau

Article

Nadeau et al J Neurophysiol. 2002 Jul . 88(1):409-21.

Insert

neuron restrictive silencer factor (Rest Mouse)

Use

Lentiviral

Tags

GFP (bicistronic with CITE/IRES)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Oct. 20, 2009

Availability

Academic Institutions and Nonprofits only

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pCas1+2

Plasmid

#72676

Purpose

Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

Depositor

Udi Qimron

Article

Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

Insert

Cas1, Cas2

Expression

Bacterial

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pJL43.1 - Pglh-2::MosTase::glh-2utr

Plasmid

#19332

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pglh-2::Mos1 transposase

Expression

Worm

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRH2521

Plasmid

#84380

Purpose

Expression of sgRNA from a imyc promoter (Pimyc)

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

sgRNA cloning site

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pESC-LEU (V5 and HA)

Plasmid

#222486

Purpose

Co-expression of two genes in yeast with V5 and HA tags and LEU2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMBP-HIS2-Vps2

Plasmid

#21494

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

VPS2 (DID4 Budding Yeast)

Tags

6x His and MBP

Expression

Bacterial

Mutation

none

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-PHLPP2

Plasmid

#22403

Depositor

Alexandra Newton

Article

Brognard et al Mol Cell. 2007 Mar 23. 25(6):917-31.

Insert

PHLPP2 (PHLPP2 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pMPRAduo:minP:GFP

Plasmid

#193739

Purpose

MPRAduo ORF (minP:GFP) donor

Depositor

Ryan Tewhey

Article

Mouri et al Cell Genom. 2022 Dec 16;3(1):100234. doi

Insert

GFP

Expression

Mammalian

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pSB3C5-phzSM

Plasmid

#141105

Purpose

Contains a phzS-phzM operon BioBrick

Depositor

Martin Buck

Article

Clifford et al Chem Sci. 2021 Jan 15;12(9):3328-3338. d

Insert

phzSM

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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FlucWT-HA-GFP11-N1

Plasmid

#91954

Purpose

Expresses FlucWT-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucWT-HA-GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-phzAG

Plasmid

#141106

Purpose

Contains a seven gene phzABCDEFG operon BioBrick

Depositor

Martin Buck

Article

Clifford et al Chem Sci. 2021 Jan 15;12(9):3328-3338. d

Insert

phzABCDEFG

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMH4-SYN-EGFP-ER

Plasmid

#22285

Depositor

Thomas Oertner

Article

Holbro et al Proc Natl Acad Sci U S A. 2009 Sep 1;106

Insert

EGFP-ER

Tags

EGFP-ER

Expression

Mammalian

Mutation

EGFP alanine 206 changed to Lysin

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

Plasmid

#231128

Purpose

Expresses xylanase via a cSAT2.0 scheme

Depositor

Xiaofeng Yang

Article

Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

Insert

xylanase

Tags

cSAT2.0

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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