We narrowed to 4,389 results for: 187

1. XLone-GvpB

   Plasmid

   #173798

   Purpose

   Mammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)

   Depositor

   Xiaojun Lian

   Article

   Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

   Insert

   GvpB
   

   Use

   Tags

   mCherry (K187N -please see depositor comments)

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G promoter

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hygro-Bem1-mCherry-AID

   Plasmid

   #173925

   Purpose

   Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

   Depositor

   Liedwij Laan

   Article

   Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

   Inserts

   Partial Bem1 C-terminus
   hygromycin marker
   Bem1 downstream sequence
   

   Use

   Tags

   mCherry- AID

   Expression

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. KBB106

   Plasmid

   #185065

   Purpose

   Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domain

   Depositor

   Ken Belanger

   Article

   Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Nup1 C-terminal region, truncated so C-terminal h…

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET11a-Z-N-wtBlc

   Plasmid

   #168258

   Purpose

   N-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip protein

   Depositor

   Jens Meiler

   Article

   Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

   Insert

   N-fragment of wtBlc + Leucine Zipper
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRBad-C-wtBlc

   Plasmid

   #168257

   Purpose

   C-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondingly

   Depositor

   Jens Meiler

   Article

   Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

   Insert

   C-fragment of wtBlc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRBad-Z-C-wtBlc

   Plasmid

   #168256

   Purpose

   C-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondingly

   Depositor

   Jens Meiler

   Article

   Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

   Insert

   Leucine Zipper + C-fragment of wtBlc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGST-POLA1-N0

   Plasmid

   #160983

   Purpose

   Express a fragment of mouse Pola1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   POLA1 (Pola1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGST-POLA1-N1-2A

   Plasmid

   #160986

   Purpose

   Express a fragment of mouse Pola1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   POLA1 (Pola1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   W84A/D88A

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGST-POLA1-N2-2A

   Plasmid

   #160987

   Purpose

   Express a fragment of mouse Pola1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   POLA1 (Pola1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   W84A/D88A

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGST-POLA1-N2

    Plasmid

    #160985

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGST-POLA1-N1

    Plasmid

    #160984

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNB689

    Plasmid

    #60133

    Purpose

    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNB756

    Plasmid

    #60149

    Purpose

    Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    YeLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    LEU1pr

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNB752

    Plasmid

    #60155

    Purpose

    Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    ADE17pr

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNB812

    Plasmid

    #60160

    Purpose

    Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    Promoter

    RNR1pr

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBA-mCherry-EGFP-PIM

    Plasmid

    #111758

    Purpose

    Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

    Depositor

    Lukas Kapitein

    Article

    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

    Insert

    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

    Promoter

    chicken beta-actin

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. EK0479 CMV-TO-VEGF-A(165) (FLP-IN)

    Plasmid

    #233384

    Purpose

    Secreted human VEGF-A

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    VEGF-A (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-ADAM10-HA

    Plasmid

    #65106

    Purpose

    Expression of HA tagged ADAM10 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-FLAG-Bbs4

    Plasmid

    #128868

    Purpose

    For mammalian expression of mouse Bbs4

    Depositor

    Aimin Liu

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

    Insert

    Bbs4 (Bbs4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-EF1a-i53

    Plasmid

    #216243

    Purpose

    Express i53 in mammalian cells as a HDR enhancer

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    i53
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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