We narrowed to 11,256 results for: NSI

1. pDONR221 SARS-CoV-2 S-frag1_nostop

   Plasmid

   #153173

   Purpose

   Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth, Mikko Taipale

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S-frag1
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   May 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pDONR223-ZNF467

   Plasmid

   #88643

   Purpose

   Donor Vector containing ZNF467 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF467 (ZNF467 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223-ZNF280C

   Plasmid

   #88658

   Purpose

   Donor Vector containing ZNF280C transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF280C (ZNF280C Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLL5.0 ShCavin1-EGFP

   Plasmid

   #187253

   Purpose

   Lentiviral expression of shRNA targeting Cavin1

   Depositor

   Alpha Yap

   Article

   Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

   Insert

   ShRNA for Cavin1 (Cavin1 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTWIN1-His6-Ssp-Httex1-37Q

   Plasmid

   #84351

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Use

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p3xFLAG CMV14 / BRAF-V600K

   Plasmid

   #131720

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   V600K

   Promoter

   CMV

   Available since

   Dec. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223-ZNF674

   Plasmid

   #88785

   Purpose

   Donor Vector containing ZNF674 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF674 (ZNF674 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223-ZNF98

   Plasmid

   #88567

   Purpose

   Donor Vector containing ZNF98 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF98 (ZNF98 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-AR-R405S

   Plasmid

   #126051

   Purpose

   Expresses human androgen receptor (R405S) in mammalian cells

   Depositor

   Elizabeth Wilson

   Article

   Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

   Insert

   AR (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R405S

   Promoter

   CMV

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTWIN1-His6-Ssp-Httex1-15Q

    Plasmid

    #84348

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Use

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223-ZNF670

    Plasmid

    #88337

    Purpose

    Donor Vector containing ZNF670 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF670 (ZNF670 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223-ZNF800

    Plasmid

    #88573

    Purpose

    Donor Vector containing ZNF800 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF800 (ZNF800 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3xFLAG CMV14 / BRAF-D594E

    Plasmid

    #131714

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D594E

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223-ZNF219

    Plasmid

    #88118

    Purpose

    Donor Vector containing ZNF219 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF219 (ZNF219 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223-ZNF830

    Plasmid

    #88342

    Purpose

    Donor Vector containing ZNF830 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF830 (ZNF830 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221-ZNF808

    Plasmid

    #101470

    Purpose

    Donor Vector containing ZNF808 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF808 (ZNF808 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221 SARS-CoV-2 S-frag6_nostop

    Plasmid

    #153184

    Purpose

    Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth, Mikko Taipale

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S-frag6
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    June 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pDONR221 SARS-CoV-2 S-frag2_nostop

    Plasmid

    #153174

    Purpose

    Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth, Mikko Taipale

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S-frag2
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    May 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDONR223-ZNF331

    Plasmid

    #88442

    Purpose

    Donor Vector containing ZNF331 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF331 (ZNF331 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223-ZNF785

    Plasmid

    #88459

    Purpose

    Donor Vector containing ZNF785 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF785 (ZNF785 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL3-321/+120 mut1&2

    Plasmid

    #35546

    Depositor

    Greg Goodall

    Article

    Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

    Insert

    promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    T to G at position -52 and T to G at position -96…

    Promoter

    none

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR223-ZNF232

    Plasmid

    #88305

    Purpose

    Donor Vector containing ZNF232 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF232 (ZNF232 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TVBB C-term-Dendra2-Puro

    Plasmid

    #169217

    Purpose

    Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked Dendra2-P2A-Puro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET41s-GST-ATF6a-AD

    Plasmid

    #64774

    Purpose

    bacterial expression of GST-tagged ATF6 (1-150)

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    ATF6a-AD (1-150) (ATF6 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-term deletion

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pDONR221-ZNF442

    Plasmid

    #101455

    Purpose

    Donor Vector containing ZNF442 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF442 (ZNF442 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNOC_hfnCas12a-Nlux_crRNA NR1

    Plasmid

    #176244

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Ribosomal subunit

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR223-ZNF366

    Plasmid

    #88019

    Purpose

    Donor Vector containing ZNF366 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF366 (ZNF366 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR221 - myc ULK1 k46I

    Plasmid

    #27624

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Expression

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pBMN-neo-Flag-TNFR1 L380A Del229-244

    Plasmid

    #44098

    Depositor

    Martin Kluger

    Article

    D'Alessio et al J Biol Chem. 2010 Jul 30;285(31):23868-7

    Insert

    human tumor necrosis factor receptor 1 (TNFRSF1A Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    L380A

    Promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pDONR223-ZNF175

    Plasmid

    #88551

    Purpose

    Donor Vector containing ZNF175 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF175 (ZNF175 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGAL4-DBD-HOXA13-58A-IDR

    Plasmid

    #145238

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13-58A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13-58A-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5-Flag-Med25-VWA

    Plasmid

    #64772

    Purpose

    Mammalian expression of Flag-tagged Med25-VWA

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VWA (MED25 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pcDNA8-Flag-LARS(1-360aa)

    Plasmid

    #139668

    Purpose

    Expresses N-teminal Flag tagged aa 1-360 of LARS1 protein

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    Leucyl-tRNA Synthetase (LARS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYPQ239-RVR

    Plasmid

    #108863

    Purpose

    FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    N623R, K629R and N633R, Cpf1 is rice codon optimi…

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p3xFLAG CMV14 / BRAF-G466V

    Plasmid

    #131722

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    G466V

    Promoter

    CMV

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDONR221 - myc ULK1 4SA

    Plasmid

    #27625

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Expression

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pGL3-321/+120 Ebox mut1

    Plasmid

    #35544

    Depositor

    Greg Goodall

    Article

    Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

    Insert

    promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    T to G at position -96 in promoter

    Promoter

    none

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGL3-321/+120 Ebox mut2

    Plasmid

    #35545

    Depositor

    Greg Goodall

    Article

    Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

    Insert

    promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    T to G at position -52 in promoter

    Promoter

    none

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCR3-vsv-AuroraB L154G/H250Y

    Plasmid

    #108490

    Purpose

    expression of vsv-AuroraB Analog sensitive (L154G/H250Y)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Analog sensitive L154G/H250Y

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Desmoplakin II no force control (FL-based)

    Plasmid

    #118724

    Purpose

    The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-FL-mCherry

    Expression

    Mammalian

    Mutation

    truncation after aa1353

    Promoter

    CMV

    Available since

    Dec. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge