We narrowed to 12,110 results for: NSI
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Plasmid#217447PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1DepositorAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only
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pGL4.23-WIPI1promotor
Plasmid#206840PurposeThe plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).DepositorInsertWIPI1 promotor (partial)
UseLuciferaseAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pINQ-ON10
Plasmid#198689PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
Tags6xHis tag, HA tag, OmpA signal peptide, and Ribos…ExpressionBacterialPromoterT7 promoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINQ-NbH4
Plasmid#198690PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody H4
Tags6xHis tag, Avitag, OmpA signal peptide, and Ribos…ExpressionBacterialPromoterT7 promoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4A 153-222
Plasmid#108256PurposeExpresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6DepositorInsertCHMP4A residues 153-222 (CHMP4A Human)
TagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP6 145-201
Plasmid#104597PurposeExpresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11DepositorAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP7 366-453
Plasmid#104598PurposeExpresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12DepositorAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH86966::AtU6p::sgRNA:NbCysP6
Plasmid#223217PurposeExpresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoterDepositorInsertNbCysP6 sgRNA
UseCRISPR and Synthetic BiologyTagsNoneExpressionPlantPromoterArabidopsis U6 promoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2_ApyD_ RBS_ApyAOHIS
Plasmid#226247PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApySDepositorInsertsHis6_ApyA
ApyD
ApyO
ApyH
ApyI
ApyS
TagsHexa-HistidineExpressionBacterialAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAT-4_Crp
Plasmid#217168PurposeMammalian expression vector for expression of hAT-4_Crp transposase (CMV promoter)DepositorInserthAT-4_Crp transposase
ExpressionMammalianAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAT-1_AMi
Plasmid#217170PurposeMammalian expression vector for expression of hAT-1_Ami transposase (CMV promoter)DepositorInserthAT-1_AMi transposase
ExpressionMammalianAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mariner-2_AMi
Plasmid#217171PurposeMammalian expression vector for expression of Mariner-2_Ami transposase (CMV promoter)DepositorInsertMariner-2_AMi transposase
ExpressionMammalianAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAT-13_AMi
Plasmid#217172PurposeMammalian expression vector for expression of hAT-13_Ami transposase (CMV promoter)DepositorInserthAT-13_AMi transposase
ExpressionMammalianAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR-CCND1shRNA3
Plasmid#220579PurposeTo inducibly knockdown CCND1 expressionDepositorAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir1-631-894
Plasmid#220089PurposeTruncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PADepositorInsertPITPNM3 (PITPNM3 Human)
TagsNeonGreen-GGSGGExpressionMammalianMutationamino acids 631-894PromoterCMVAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1_oROS-HT_LF(C199S)
Plasmid#216412PurposeExpresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.DepositorInsertoROS-HT_LF(C199S)
ExpressionMammalianPromoterCMVAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1-lifeact-oROS-HT
Plasmid#216420PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.DepositorInsertlifeact-oROS-HT
ExpressionMammalianPromoterCMVAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-N1-Nir1-613-630
Plasmid#220088PurposeN-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PADepositorInsertPITPNM3 (PITPNM3 Human)
TagsGGSGG-NeonGreenExpressionMammalianMutationamino acids 613-630PromoterCMVAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-C Sars-CoV-2 E P71L
Plasmid#194804PurposeSars-CoV-2 E_P71L in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 N_D3L
Plasmid#194806PurposeSars-CoV-2 N_D3L in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 N_D63G
Plasmid#194807PurposeSars-CoV-2 N_D63G in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 N_R203M
Plasmid#194810PurposeSars-CoV-2 N_R203M in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 N_S235F
Plasmid#194811PurposeSars-CoV-2 N_S235F in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-C Sars-CoV-2 N_T205I
Plasmid#194812PurposeSars-CoV-2 N_T205I in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIRHDGAL43C
Plasmid#218279PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
ExpressionYeastMutationGAL3 (S509P), DHFR (P66L)Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHDGAL3C
Plasmid#218276PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
ExpressionYeastMutationGAL3 (S509P), DHFR (P66L)Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AtSLAC1 WT
Plasmid#212946PurposeVector used for expression of AtSLAC1 WT in mammalian cellDepositorAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AtSLAC1 6D
Plasmid#212947PurposeVector used for expression of AtSLAC1 6D in mammalian cellDepositorInsertSlow anion channel-associated 1 (OZS1 Mustard Weed)
ExpressionMammalianMutationT62D/S65D/S107D/S124D/S146D/S152DPromoterCMVAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-hALDOA D34S
Plasmid#210813PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
UseAdenoviralTagsHAMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA K230A
Plasmid#210811PurposeTo block the formation of the initial Schiff base with FBPDepositorInsertALDOA (ALDOA Human)
TagsHAExpressionMammalianMutationresidues 230 of ALDOA mutated to alaninePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hALDOA D34S
Plasmid#210807PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
UseLentiviralTagsHAMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_6F→A
Plasmid#194269PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→ADepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationF276A, F283A, F289A, F367A, F397A, F401APromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→A_CR
Plasmid#194263PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CRDepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationM322A, M323A, M336A, M337A, M339A mutations in TD…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→A_IDR
Plasmid#194264PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to AlanineDepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationM307A, M311A, M359A, M405A, M414A mutations in TD…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→L_IDR
Plasmid#194265PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucineDepositorInsertTDP-43 (TARDBP Human)
Tags6XHistagExpressionBacterialMutationM307L, M311L, M359L, M405L, M414L mutations in TD…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→Y_IDR
Plasmid#194266PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosineDepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationM307Y, M311Y, M359Y, M405Y, M414Y mutations in TD…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5A→M_IDR
Plasmid#194267PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionineDepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationA297M, A367M, A381M, A388M, A391M mutations in TD…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_CRF0.5
Plasmid#208657PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in neuronsDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
UseAAVPromoterhSynAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-tagged Sox17FNV
Plasmid#206391PurposeExpresses Halo-tagged mouse SOX17FNV in mammalian cells, for single-molecule tracking.DepositorAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-mBrn2-3xflag
Plasmid#206390PurposeExpresses 3xflag fused mouse BRN2 in mammalian cells, for lentivirus generation.DepositorAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only