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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,261 results for: 338

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Showing: 3941 - 3960 of 6261 results

  1. pTS2312

    Plasmid
    #169622
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169622-plasmid-map-sequence-id-365612

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  2. pTS2317

    Plasmid
    #169624
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169624-plasmid-map-sequence-id-365608

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  6. pCAG1.1-Armc12/FLAG

    Plasmid
    #180494
    Purpose
    Expression vector of mouse armadillo repeat containing 12 (Armc12) tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.
    Depositor
    Masahito Ikawa
    Article
    Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118
    Insert
    armadillo repeat containing 12 (Armc12 Mouse)
    Tags
    FLAG tag
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180494-plasmid-map-sequence-id-356913

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  7. S+Q111R

    Plasmid
    #167173
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+Q111R
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167173-plasmid-map-sequence-id-334482

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  8. S+N122D

    Plasmid
    #167174
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+N122D
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed N at 111 to D
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167174-plasmid-map-sequence-id-334489

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  10. GFP-Tac-STC

    Plasmid
    #162490
    Purpose
    Expresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its stem region, TM…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162490-plasmid-map-sequence-id-323468

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  19. LSB-hsa-miR-675-3p

    Plasmid
    #103720
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-675-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-675-3p target (MIR675 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103720-plasmid-map-sequence-id-196488

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  20. LSB-hsa-miR-675-5p

    Plasmid
    #103721
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-675-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-675-5p target (MIR675 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103721-plasmid-map-sequence-id-196490

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Showing: 3941 - 3960 of 6261 results