N-Terminal Split Cas9 D10A Nickase with GyrA intein
Plasmid
#58695
Purpose
Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
Depositor
Insert
D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
Use
AAV and CRISPR
Tags
GyrA Nsplit Intein, HA Tag, and NLS
Expression
Mammalian
Mutation
D10A nickase converting mutation to SpCas9
Promoter
CBh
Available since
Sept. 25, 2014
Availability
Academic Institutions and Nonprofits only
