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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 77 results for: 20733

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Showing: 21 - 40 of 77 results

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  2. pBS7

    Plasmid
    #83774
    Purpose
    Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    Venus
    Tags
    Venus
    Expression
    Yeast
    Mutation
    F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    83774-plasmid-map-sequence-id-159984

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  3. pDH6

    Plasmid
    #83777
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    YFP
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y mutations added.
    Available Since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83777-plasmid-map-sequence-id-160009

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  4. pDH5

    Plasmid
    #83775
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; his selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    YFP
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…
    Available Since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83775-plasmid-map-sequence-id-159997

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  9. pDH3

    Plasmid
    #83789
    Purpose
    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    CFP
    Tags
    CFP
    Expression
    Yeast
    Mutation
    F64L, Y66W, N146I, M153T, V163A, N164H mutations …
    Available Since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83789-plasmid-map-sequence-id-160016

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  16. ATP1A1_G8_Dual_sgRNA

    Plasmid
    #178104
    Purpose
    Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178104-plasmid-map-sequence-id-350193

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  17. ATP1A1_G6_T804N_Dual_pegRNA

    Plasmid
    #173200
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173200-plasmid-map-sequence-id-339515

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  18. eSpCas9(1.1)_No_FLAG_NPM1_G6

    Plasmid
    #178091
    Purpose
    Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178091-plasmid-map-sequence-id-350141

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  19. ATP1A1_G4_Q118R_EBFP_to_EGFP_Dual_pegRNA

    Plasmid
    #178100
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and EBFP_To_EGFP pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178100-plasmid-map-sequence-id-350130

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Showing: 21 - 40 of 77 results