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Plasmid#128741PurposeExpression of histone H3.3K4M-FLAG/HADepositorInsertH3.3K4M (H3-3A Human)
UseRetroviralTagsFLAG and HAExpressionMutationK4MPromoterMSCVAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-H3.3-YFP
Plasmid#8693DepositorInsertH3.3A (His3.3A Fly)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pQCXIpuro-H3.3K4M-FLAG/HA
Plasmid#128742PurposeExpression of histone H3.3K4M-FLAG/HADepositorInsertH3.3K4M (H3-3A Human)
UseRetroviralTagsFLAG and HAExpressionMutationK4MPromoterCMVAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
HS-H3.3A d436-GFP
Plasmid#8697DepositorInsertH3.3A truncated (His3.3A Fly)
UseTagsGFPExpressionInsectMutationDeletion of amino acids 4-36.PromoterAvailable sinceJune 30, 2005AvailabilityAcademic Institutions and Nonprofits only -
CENPW H3.3 gRNA
Plasmid#90620Purpose3rd generation lentiviral gRNA plasmid targeting human CENPWDepositorInsertCENPW (Guide Designation H3.3)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-H3.3K27M-FLAG-BFP
Plasmid#209434PurposeLentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-K27M with BFPDepositorInsertH3.3-K27M
UseTagsExpressionMammalianMutationK27MPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-H3.3-WT-FLAG-BFP
Plasmid#206139PurposeExpression of a FLAG tagged human histone H3.3 gene with BFPDepositorInsertH3.3 (H3-3A Human)
UseTagsFLAG-eBFPExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKT-H3.3-K27M-katushka
Plasmid#209443PurposeSB-transposable plasmid expressing a mutant histone H3.3-K27M in tandem with Katushka (RFP)DepositorInsertH3.3-K27M
UseTagsExpressionMammalianMutationK27MPromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-H3.3-polyA
Plasmid#59780PurposeExpress Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertH3F3B (H3-3B Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIHEU_miniSOG2 T2A H3.3A-EGFP
Plasmid#87411PurposeUAS vector to express Histone 3.3A GFP fusion and miniSOG2 in DrosophilaDepositorInsertmini singlet oxygen generator 2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBT346.6-H3.3-K4M-bGHpolyA
Plasmid#128743PurposeInducible expression of histone H3.3K4M-FLAG/HADepositorInsertH3.3K4M (H3-3A Human)
UseTagsFLAG and HAExpressionMammalianMutationK4MPromoterCAGAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(WT)-3AID-HA-2A-mTurquoiseBSD
Plasmid#225701PurposeLentiviral expression of AID degron tagged Wildtype H3.3 and mTurquoise fused BlasticidinRDepositorUseLentiviralTags3xAID HA and T2A-mTurquoiseExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD
Plasmid#225697PurposeLentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
px459-H3.3 (H3f3b) sgRNA
Plasmid#186931PurposeH3f3b sgRNA used for genomic targeting at H3f3b C-terminalDepositorInsertH3f3b sgRNA (H3f3b Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterU6Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRES-H3.3-G34R-FLAG-BFP
Plasmid#206140PurposeExpression of a FLAG tagged human histone mutant H3.3-G34R with BFPDepositorInsertH3.3 (H3-3A Human)
UseTagsFLAG-eBFPExpressionMammalianMutationG34RPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-H3.3-G34R-FLAG-BFP
Plasmid#209440PurposeLentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-G34R with BFPDepositorInsertH3.3-G34R
UseTagsExpressionMammalianMutationG34RPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Anti-Histone H3.3 [N158/28R]
Plasmid#182106PurposeMammalian Expression Plasmid of anti-Histone H3.3 (Rat). Derived from hybridoma N158/28.DepositorInsertanti-Histone H3.3 (Rattus norvegicus) recombinant mouse monoclonal antibody (H3f3b Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD
Plasmid#225703PurposeLentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mTurquoiseExpressionMammalianMutationK36MPromoterpEF1AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD
Plasmid#225702PurposeLentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mTurquoiseExpressionMammalianMutationK27MPromoterpEF1AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD
Plasmid#225699PurposeLentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationK36MPromoterpEF1AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only