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We narrowed to 209 results for: LOV2

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Showing: 21 - 40 of 209 results

  3. pMRX-IP-EGFP-LOV2-Cyb5a

    Plasmid
    #233348
    Purpose
    Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N538E and N-terminus is deleted (Cyb5a 95-134)
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233348-plasmid-map-sequence-id-466824

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  5. pMRX-IP-EGFP-LOV2-TMEM106B

    Plasmid
    #233347
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-TMEM106B in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N538E and N-terminus is deleted (TMEM106B 90-274)
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233347-plasmid-map-sequence-id-469408

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  6. pTriEx-mVenus-PA-Rac1-C450A

    Plasmid
    #22021
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Publication
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains the light-insensitive C450A m…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  7. pEB1N-LZ-mCherry-LOV2(wt)

    Plasmid
    #107693
    Purpose
    N-terminal half of π-EB1 with wild-type LOV2, mCherry-tagged to track microtubule ends
    Depositor
    Torsten Wittmann
    Publication
    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPRE1 (MAPRE1 Human)
    Tags
    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…
    Expression
    Mammalian
    Mutation
    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107693-plasmid-map-sequence-id-209378

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  9. PI(LM)-Rac1(WT)dc1.FLARE

    Plasmid
    #87590
    Purpose
    PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor
    Depositor
    Klaus Hahn
    Publication
    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LOV2
    PBD
    Rac1
    Tags
    YPet and dTurq
    Expression
    Bacterial, Insect, and Mamm…
    Promoter
    CMV
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    87590-plasmid-map-sequence-id-177184

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  10. pMRX-IP-EGFP-LOV2-OMP25

    Plasmid
    #233346
    Purpose
    Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N538E and N-terminus is deleted
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233346-plasmid-map-sequence-id-469428

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  13. pNUT1

    Plasmid
    #175746
    Purpose
    Single-component photo-inducible (LOV2-based) nucleolus-targeting tool 1
    Depositor
    Yubin Zhou
    Publication
    Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-LOV2-NoLS (HIV Rev transactivating protein)
    Tags
    mCherry
    Expression
    Mammalian
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    175746-plasmid-map-sequence-id-361255

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  15. pMRX-IP-EGFP-PA-BAX(ER)

    Plasmid
    #228941
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N538E, N-terminus and C-terminus are deleted. BAX…
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228941-plasmid-map-sequence-id-469413

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  17. pMRX-IP-EGFP-PA-BAX(lyso)

    Plasmid
    #228939
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N538E, N-terminus and C-terminus are deleted. BAX…
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228939-plasmid-map-sequence-id-469416

    Enlarge

  18. pMRX-IP-EGFP-PA-BAX(ER, G108V)

    Plasmid
    #233344
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-Cyb5a in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    BAX(3-171), N-terminus and C-terminus are deleted…
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    233344-plasmid-map-sequence-id-474567

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  19. pMRX-IP-EGFP-PA-BAX(lyso, G108V)

    Plasmid
    #233345
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    BAX(3-171), N-terminus and C-terminus are deleted…
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233345-plasmid-map-sequence-id-469414

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  20. pMRX-IP-EGFP-PA-BAX(mito)

    Plasmid
    #228942
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N538E, N-terminus and C-terminus are deleted. BAX…
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228942-plasmid-map-sequence-id-466826

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Showing: 21 - 40 of 209 results