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We narrowed to 611 results for: SPY

Showing: 21 - 40 of 611 results

  7. pET21 MBPx-SpyCatcher

    Plasmid
    #72327
    Purpose
    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET21 MBPx-SpyCatcher
    Use
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Mutation
    MBP contains A312V, I317V mutations and 172, 173,…
    Promoter
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72327-plasmid-map-sequence-id-434061

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  9. pIBA3-SpyCatcher-sfGFP

    Plasmid
    #107420
    Purpose
    For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    SpyCatcher
    Use
    Tags
    His tag, StrepII, and superfolder GFP
    Expression
    Bacterial
    Mutation
    Promoter
    Tet
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107420-plasmid-map-sequence-id-207599

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  11. Dead Streptavidin-SpyCatcher

    Plasmid
    #59547
    Purpose
    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead-dloop-Streptavidin-SpyCatcher
    Use
    Tags
    N-terminally shortened SpyCatcher
    Expression
    Bacterial
    Mutation
    PolyAsp in L3/4 loop for ion-exchange
    Promoter
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Use
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pCG-D-mSpyCatcher-His6-E

    Plasmid
    #87376
    Purpose
    An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF ass
    Depositor
    Tom Ellis
    Article
    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021
    Insert
    mSpyCatcher
    Use
    Synthetic Biology
    Tags
    His6 tag
    Expression
    Mutation
    Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87376-plasmid-map-sequence-id-183395

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  14. pCG-D-SpyTag-His6-E

    Plasmid
    #87375
    Purpose
    An ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assembly
    Depositor
    Tom Ellis
    Article
    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021
    Insert
    SpyTag
    Use
    Synthetic Biology
    Tags
    His6 tag
    Expression
    Mutation
    Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87375-plasmid-map-sequence-id-183404

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  15. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid
    #107421
    Purpose
    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    SpyCatcher
    Use
    Tags
    His tag, StrepII, and superfolder GFP
    Expression
    Bacterial
    Mutation
    mutation of glutamate 77 to glutamine
    Promoter
    Tet
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107421-plasmid-map-sequence-id-207622

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  16. pIBA2-SpyTag-MBP

    Plasmid
    #107422
    Purpose
    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    Maltose-binding protein (MalE)
    Use
    Tags
    OmpA signal peptide, SpyTag, and StrepII
    Expression
    Bacterial
    Mutation
    Promoter
    Tet
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107422-plasmid-map-sequence-id-212955

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  19. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid
    #72325
    Purpose
    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-mEGFP-SpyTag
    Use
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72325-plasmid-map-sequence-id-137259

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Showing: 21 - 40 of 611 results