-
Plasmid#55434PurposeLocalization: Cytoskeleton, Excitation: 433, Emission: 475DepositorInsertMyotilin (MYOT Human)
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-STIL
Plasmid#136795PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tagDepositorInsertSNAP-STIL (STIL Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac GST-STIL
Plasmid#80265Purposebaculovirus creation plasmid for GST-STILDepositorInsertSTIL (STIL Human)
UseTagsGSTExpressionInsectMutationPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-STIL
Plasmid#136844PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to CLIP-tagDepositorInsertCLIP-STIL (STIL Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
mGeos2-VEL-Myotilin-C-14
Plasmid#57554PurposeLocalization: Cytoskeleton, Excitation: 503 / 569, Emission: 514 / 581DepositorInsertMyotilin (MYOT Human)
UseTagsmGeos2-VELExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5 FRT/TO MycLAP-STIL
Plasmid#80266Purposemammalian expression plasmid for MycLAP-STILDepositorInsertSTIL (STIL Human)
UseTagsMyc-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO MycLAP-STIL 5A
Plasmid#80267Purposemammalian expression plasmid for MycLAP-STIL 5ADepositorInsertSTIL (STIL Human)
UseTagsMyc-GFPExpressionMammalianMutationS1103A, S1108A, S1111A, S1116A, T1119APromoterCMVAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO MycLAP-STIL ∆CC
Plasmid#80268Purposemammalian expression plasmid for MycLAP-STIL coiled-coil deletionDepositorInsertSTIL (STIL Human)
UseTagsMyc-GFPExpressionMammalianMutationmissing conserved coiled-coil (amino acids 721-74…PromoterCMVAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)
Plasmid#149276PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorInsertModified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)
Plasmid#217802PurposeVariant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)
Plasmid#217796PurposeVariant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.DepositorInsertCdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
B. subtilis CRISPRi Essential Gene Knockdown Library
Plasmid Kit#1000000116PurposeB. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.DepositorAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
B. subtilis Single Gene Deletion Library--Kanamycin
Plasmid Kit#1000000115PurposeB. subtilis Single Gene Deletion Library - Kanamycin: Arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.DepositorAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)
Plasmid#217797PurposeVariant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.DepositorInsertCdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationCdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)
Plasmid#217803PurposeVariant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPIP_PepTile_40merFragments_65CancerDrivers
Pooled Library#172109PurposeLentiviral peptide expression library for overexpression fitness screeningDepositorUseLentiviralAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
KSHV Tiling Library
Pooled Library#180272PurposesgRNA library tiling entire ~154 kb of the BAC16 Kaposi’s Sarcoma-associated Herpes viral genome. A guide every ~8 bp allows pooled screening of viral genome.DepositorExpressionMammalianSpeciesKaposi’s sarcoma-associated herpes virusUseLentiviralAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRi-v2, Mitochondria, Trafficking, and Motility (h4), top 5 sgRNAs/gene
Pooled Library#83974PurposeHuman CRISPRi Pooled Library targeting Mitochondria, Trafficking, and Motility genesDepositorAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRa-v2, Mitochondria, Trafficking, and Motility (h4), top 5 sgRNAs/gene
Pooled Library#83983PurposeHuman CRISPRa Pooled Library targeting Mitochondria, Trafficking, and Motility genesDepositorAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCRISPRa-v2, Mitochondria, Trafficking, and Motility (m4), top 5 sgRNAs/gene
Pooled Library#84001PurposeMouse CRISPRa Pooled Library targeting Mitochondria, Trafficking, and Motility genesDepositorAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only