Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,147 results for: transfer

Showing: 571 - 600 of 10147 results

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pET-SOMA

Plasmid

#118935

Purpose

Used to express the SOMA FRET sensor in E. coli.

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES

Plasmid

#175811

Purpose

The lentiviral vector for Booster-PKA, a Red-Shifted genetically encoded FRET biosensor for monitoring PKA activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-SCW-HHL

Plasmid

#190627

Purpose

Bacterial expression of genetically encoded calcium indicator SCW-HHL

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW-HHL

Expression

Bacterial

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-SPW-HHL

Plasmid

#190629

Purpose

Bacterial expression of genetically encoded protease indicator SPW-HHL

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SPW-HHL

Expression

Bacterial

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Py2087ACR1

Plasmid

#153032

Purpose

Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

Insert

Py2087ACR1

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-Y100F

Plasmid

#110826

Purpose

Bacterial expression of E.coli DHFR Y100F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

Insert

ecDHFR

Expression

Bacterial

Mutation

Y100 mutated to F100

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSIN-FHA2 R605A-Ypet-34-ECFP-hLaminA

Plasmid

#235123

Purpose

FHA2-mutated FRET biosensor R605A; FRET biosensor for live cell visualization of pSer22 Lamin A

Depositor

Qin Peng

Article

Liu et al Biomater Res. 2024 Oct 22;28:0091. doi:

Insert

FHA2 R605A-Ypet-34-ECFP-hLaminA

Use

Lentiviral

Expression

Mammalian

Mutation

FHA2 R605A

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(E502Q)

Plasmid

#226973

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(E502Q)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

E502Q

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD-AE

Plasmid

#226982

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD-AE

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD_beta-gamma

Plasmid

#226981

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD_beta-gamma

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(W392A)

Plasmid

#226980

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(W392A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

W392A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(W392F)

Plasmid

#226979

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(W392F)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

W392F

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(F401A)

Plasmid

#226978

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(F401A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

F401A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(L616E)

Plasmid

#226977

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(L616E)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

L616E

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(R226K)

Plasmid

#226976

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226K)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226K

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(R226M)

Plasmid

#226975

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226M)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226M

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(R226E)

Plasmid

#226974

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226E)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226E

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(H514E)

Plasmid

#226972

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514E)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514E

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(H514A)

Plasmid

#226971

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(C500S)

Plasmid

#226970

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(C500S)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

C500S

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(G853A)

Plasmid

#226969

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(G853A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

G853A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD-AE

Plasmid

#226968

Purpose

Expressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD-AE

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR/eCas9-GPIDAF

Plasmid

#213710

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

CRISPR

Tags

3XFLAG and Twin-strep-tag

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR/eCas9-GPIBY55

Plasmid

#213709

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

CRISPR

Tags

3XFLAG and Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV/CaMKIIa::iLMO2

Plasmid

#129481

Purpose

AAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoter

Depositor

Robert Gross

Article

Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

Insert

iLMO2

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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tdTomato-β-actin3'UTR2

Plasmid

#183717

Purpose

tdTomato with fragment nucleotides 441-676 of beta-actin 3'UTR inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 441-676) of mouse Actb 3'UTR

Use

Lentiviral

Promoter

UbiC

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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tdTomato--β-actin CDS2

Plasmid

#183714

Purpose

tdTomato with fragment nucleotides 398-818 of beta-actin coding sequence inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 398-818) of mouse Actb coding dsequence

Use

Lentiviral

Promoter

UbiC

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
tdTomato-β-actin3'UTR1

Plasmid

#183716

Purpose

tdTomato with fragment nucleotides 1-440 of beta-actin 3'UTR inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 1-440) of mouse Actb 3'UTR

Use

Lentiviral

Promoter

UbiC

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N0

Plasmid

#160983

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N2

Plasmid

#160985

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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