-
-
pFRT-TO-DEST
Plasmid#106348PurposeDestination vector for gateway cloning. Developed for Flp-In T-REx system.DepositorTypeEmpty backboneUseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMO746
Plasmid#117480Purposeupp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanRDepositorInserturacil phosphoribosyltransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg7_T
Plasmid#147556PurposeMammalian Expression of HsSmg7DepositorInsertHsSmg7 (SMG7 Human)
UseTagsExpressionMammalianMutationtwo silent mutation G216A, A1521G and one non sil…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot10
Plasmid#79254PurposeExpresses EGFP-tagged DmNot10 in S2 cellsDepositorInsertDmNot10 (Not10 Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot11
Plasmid#79253PurposeExpresses EGFP-tagged DmNot11 in S2 cellsDepositorInsertDmNot11 (Not11 Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-466
Plasmid#92251PurposePlasmid for expression of GST-tagged human TNRC6C CED in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsGSTExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690PromoterEF1AAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40
Plasmid#79251PurposeExpresses EGFP-tagged DmCaf40 in S2 cellsDepositorInsertDmCaf40 (Rcd-1 Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1016
Plasmid#92255PurposePlasmid for expression of HA-tagged human TNRC6C CED in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsHAExpressionMammalianMutation.C-terminal effector domain (CED): aa 1369-1690PromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-133
Plasmid#92256PurposePlasmid for expression of NHA-tagged human TNRC6C CED in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsNHAExpressionMammalianMutation.C-terminal effector domain (CED): aa 1369-1690PromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-SD-S1616A/S1691A
Plasmid#215909PurposeExpression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationcontain aa1224-1709, S1616A, S1691APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1332D/S1346D
Plasmid#215912PurposeExpression of full-length TNRC6A with S1332D/S1346D mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1332D, S1346DPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1332A/S1346A
Plasmid#215913PurposeExpression of full-length TNRC6A with S1332A/S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1332A, S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1616D/S1691D
Plasmid#215914PurposeExpression of full-length TNRC6A with S1616D/S1691D mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1616D, S1691DPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1616A/S1691A
Plasmid#215915PurposeExpression of full-length TNRC6A with S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1616A, S1691APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A
Plasmid#215900PurposeExpression of TNRC6A-CIM1 domain with S1332A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1528, S1332APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1346A
Plasmid#215901PurposeExpression of TNRC6A-CIM1 domain with S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1528, S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A/S1346A
Plasmid#215902PurposeExpression of TNRC6A-CIM1 domain with S1332A/S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1528, S1332A/S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A
Plasmid#215903PurposeExpression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1709, S1332A, S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-S1616A/S1691A
Plasmid#215904PurposeExpression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1709, S1616A, S1691APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only
