-
Plasmid#67631PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.DepositorInsertMBII85 snoRNA
UseTagsExpressionMammalianMutationmutation in 5'ssPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemTagsExpressionMammalianMutationY64L (mutRFP)PromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT2 NCmut-msfGFP
Plasmid#180320Purposemammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFPDepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT2 F20D, V27DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 _3xHA-Ago2_CATmut
Plasmid#73540PurposeInducible lentiviral expression of Ago2_CATmutDepositorInsertAgo2 (Ago2 Mouse)
UseLentiviralTags3xHAExpressionMammalianMutationCatalytic mutant PIWI domain D670APromoterTRE promoter, Tet ONAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
TBRE-TK-Luc-MUT
Plasmid#36245DepositorInsertTwo copies of the Brachyury T-box site
UseTagsLuciferaseExpressionMammalianMutationMutations in the Brachyury palindromic element:CA…PromoterTK minimal promoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 899
Plasmid#194193PurposeIncludes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutationmutated KLF binding sites (position 899)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
LUC45 (L/Sec/mutP)
Plasmid#112148PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U) and SE…PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NPYmut
Plasmid#208679PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in neuronsDepositorInsertGPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2-Mcl-1 mut
Plasmid#19133DepositorInsertMcl-1 promoter (MCL1 Human)
UseLuciferaseTagsExpressionMammalianMutationNFkB binding-site mutant, the sequence GGGGTCTTCC…PromoterAvailable sinceOct. 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-CXXCmut-pEF
Plasmid#127895PurposeExpresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAADepositorInsertTET3-CxxCmut (TET3 Human)
UseTagsHA and FlagExpressionMammalianMutationPromoterEF-1aAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
EE-mut SV40 luc
Plasmid#8460DepositorInserthuman cyclin E promoter fragment, mutant (CCNE1 Human)
UseLuciferaseTagsExpressionMammalianMutationE2F sites I, II, and III mutated.PromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV_mApple-SEPT7 Gmut2
Plasmid#180317Purposemammalian expression of human SEPT7 Gmut2 fused to mAppleDepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsmAppleExpressionMammalianMutationSEPT7 H279DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-CDX2-MUT
Plasmid#16554DepositorInsertCDX2 (CDX2 Human)
UseAdenoviralTagsExpressionMammalianMutationRKO mutationPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTCF79 H-2D(b) (CD8 mut)
Plasmid#180453PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(b) (CD8 mut)
UseTagsBSP41ExpressionBacterialMutationD227K, replacement of transmembrane domain with e…PromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
-227 hTF (mutant NF-kB)
Plasmid#15443DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationNF-kappaB binding domain mutatedPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pWUR 1+2 tetO mut89
Plasmid#80102PurposeEncodes a set of wild-type Cas1+2 and a mutant set of Cas1+2DepositorUseTagsExpressionBacterialMutationQ24H, P202Q, G241D, E276D, L297QPromoterAvailable sinceDec. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKEN GFP mut3
Plasmid#20410DepositorInsertGFP
UseTagsExpressionBacterialMutationS65G S72APromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCFL-mPML-3KRmut-iB
Plasmid#140287PurposeExpression of mouse PML KR mutantDepositorInsertPML (Pml Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 10 mut B-Myb
Plasmid#25966DepositorInsertB-Myb (Mybl2 Mouse)
UseTagsFlagExpressionMammalianMutationchanged threonines 267, 408, 497, 519, 522 and 52…PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEB1-2ndVal mGFPmut2
Plasmid#104033PurposePlasmid encoding 2ndVal mGFPmut2DepositorInsert2ndVal mGFPmut2
UseLow copyTagsExpressionBacterialMutationmGFPmut2 with valine inserted in 2nd positionPromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only