Addgene

We narrowed to 13,827 results for: NTS

Showing: 381 - 400 of 13827 results

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pGL3-LRR

Plasmid

#66937

Purpose

Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Reverse orientation (attR2, attR1).

Depositor

Alvaro Monteiro

Article

Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV-40

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA

Plasmid

#87584

Purpose

PV5 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAM5089

Plasmid

#85125

Purpose

KaiB expressed from native promoter from NSII-Nt. Complements rhythms of kaiB null strain.

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

PkaiB-kaiB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBW829_pCAG-pExpr-LUT

Plasmid

#87554

Purpose

Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#87560

Purpose

Expresses aa397-423 of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa397-423

Use

Cre/Lox and Synthetic Biology

Tags

PYL

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

Plasmid

#87559

Purpose

Expresses aa1-396 of Flp recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa1-396

Use

Cre/Lox and Synthetic Biology

Tags

ABI

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-C. thermophilum LIC

Plasmid

#74780

Purpose

encodes GST-tagged dynein light intermediate chain from Chaetomium thermophilum

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

dynein light intermediate chain (Chaetomium thermophilum)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpA

Plasmid

#87587

Purpose

PV8 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pYO411

Plasmid

#235732

Purpose

Protein expression of ZZ-FL yeast Atg14 in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ATG14

Use

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO69

Plasmid

#235747

Purpose

Protein expression of FL ScVPS34 in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Use

Tags

Expression

Yeast

Mutation

S2A

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO361

Plasmid

#235748

Purpose

Protein expression of ScVPS34 HELCAT

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 HELCAT

Use

Tags

Expression

Yeast

Mutation

aa 268-875

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO227

Plasmid

#235749

Purpose

Protein expression of ZZ-FL ScVPS30

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS30

Use

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO832

Plasmid

#235755

Purpose

Protein expression of ScVPS30 (1-215E), untagged

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ScVPS30

Use

Tags

Expression

Yeast

Mutation

aa 1-215E

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-M2

Plasmid

#225735

Purpose

To express M2 (219-399) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 219-399

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMV261-igr3K253E

Plasmid

#225739

Purpose

To express igr3 (Rv3542c-Rv3541c-Rv3540c) with K253E mutation in Rv3542c in Mycobacteria

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

Myc-igr3K253E

Use

Tags

c-Myc

Expression

Bacterial

Mutation

igr3 K253E

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMV261-igr3WT

Plasmid

#225738

Purpose

To express wild type igr3 (Rv3542c-Rv3541c-Rv3540c) in Mycobacteria

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

Myc-igr3WT

Use

Tags

c-Myc

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-M1

Plasmid

#225734

Purpose

To express M1 (13-193) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 13-193

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRPL28 SpHIS5 (pNTI663)

Plasmid

#115433

Purpose

Expresses wild-type RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Use

Tags

Expression

Yeast

Mutation

MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRPL28(Q38P) SpHIS5 (pNTI664)

Plasmid

#115434

Purpose

Expresses Q38P RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Use

Tags

Expression

Yeast

Mutation

Q38P; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRPL28(H39L) SpHIS5 (pNTI665)

Plasmid

#115435

Purpose

Expresses H39L RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Use

Tags

Expression

Yeast

Mutation

H39L; MfeI site in intron

Promoter

ashbya gospii TEF1 and endogenous

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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