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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 415 results for: SaCas9

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Showing: 381 - 400 of 415 results

  5. Mammalian_SaAID3S

    Plasmid
    #188651
    Purpose
    Expresses SaAID3S and gRNA in mammalian cells
    Depositor
    Keiji Nishida
    Publication
    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    nSaCas9-AID3S--UGI
    Sa-sgRNA
    Tags
    UGI
    Expression
    Mammalian
    Mutation
    D10A for SaCas9
    Promoter
    EF-1a and H1
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188651-plasmid-map-sequence-id-375883

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  6. Mammalian_SaAID2S

    Plasmid
    #188652
    Purpose
    Expresses SaAID2S and gRNA in mammalian cells
    Depositor
    Keiji Nishida
    Publication
    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    AID2S-nSaCas9--UGI
    Sa-sgRNA
    Tags
    UGI
    Expression
    Mammalian
    Mutation
    D10A for SaCas9
    Promoter
    EF-1a and H1
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188652-plasmid-map-sequence-id-375885

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  9. SauKKHCas9 PE2*

    Plasmid
    #169852
    Purpose
    Mammalian Expression, sauCas9-KKH based prime editor
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9KKH-N580A-M-MLV
    Tags
    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169852-plasmid-map-sequence-id-337167

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  10. pAAV/CMV-SCLM

    Plasmid
    #216758
    Purpose
    AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon
    Depositor
    George Augustine
    Insert
    SuperClomeleon followed by WPRE and human growth hormone polyA terminator
    Use
    AAV
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    CMV (cytomegalovirus)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216758-plasmid-map-sequence-id-429489

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  14. pEPOR1CB0113

    Plasmid
    #117552
    Purpose
    Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117552-plasmid-map-sequence-id-228090

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  16. AAV-CMV-scFVDnmt3AL_bGHpA

    Plasmid
    #177347
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Publication
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177347-plasmid-map-sequence-id-353618

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  17. AAV-CMV-scFVtet2_bGHpA

    Plasmid
    #177352
    Purpose
    AAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Publication
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    Catalytic domains of human TET2 (1129–1936 and 14…
    Promoter
    CMV promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177352-plasmid-map-sequence-id-353673

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  18. pAAV-BiSSTe4-dSaCa9-KRAB(Zim3)-pA

    Plasmid
    #255031
    Purpose
    AAV plasmid encoding a dSaCas9-KRAB(Zim3) fusion gene controlled from an SST-specific neuronal enhancer, BiSSTe4
    Depositor
    Jonathan Ploski
    Insert
    dSaCas9-KRAB(Zim3)
    Use
    AAV and CRISPR
    Available since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    255031-plasmid-map-sequence-id-509446

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  19. pEPOR2KN0078

    Plasmid
    #117645
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Expression
    Plant
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    117645-plasmid-map-sequence-id-240245

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  20. pEPOR2KN0080

    Plasmid
    #117647
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Expression
    Plant
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    117647-plasmid-map-sequence-id-228399

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Showing: 381 - 400 of 415 results