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We narrowed to 1,248 results for: cas12

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Showing: 381 - 400 of 1248 results

  4. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid
    #140200
    Purpose
    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    dLbCas12a crRNA scaffold
    Promoter
    PgpdA
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140200-plasmid-map-sequence-id-310179

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  8. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid
    #114070
    Purpose
    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114070-plasmid-map-sequence-id-220511

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  9. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197026
    Purpose
    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Expression
    Mammalian
    Mutation
    D52R / T132R
    Promoter
    CBh, CMV, hU6
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197026-plasmid-map-sequence-id-394112

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  10. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197027
    Purpose
    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enRhCas12f1
    Expression
    Mammalian
    Mutation
    L270R
    Promoter
    CBh, CMV, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197027-plasmid-map-sequence-id-388964

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  12. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197024
    Purpose
    Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized OsCas12f1
    Expression
    Mammalian
    Promoter
    CBh, CMV, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197024-plasmid-map-sequence-id-388957

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  14. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

    Plasmid
    #114069
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114069-plasmid-map-sequence-id-220520

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  15. pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

    Plasmid
    #140197
    Purpose
    Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    dLbCas12a-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNase deactivated
    Promoter
    PgpdA
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140197-plasmid-map-sequence-id-309708

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  17. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid
    #140193
    Purpose
    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNAse deactivated
    Promoter
    PgpdA and PtrpC
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140193-plasmid-map-sequence-id-309648

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  18. pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197025
    Purpose
    Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized RhCas12f1
    Expression
    Mammalian
    Promoter
    CBh, CMV, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197025-plasmid-map-sequence-id-388959

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Showing: 381 - 400 of 1248 results