We narrowed to 3,493 results for: cgas

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SL2-sgTelo-MTSa/EGFP/pdCas9-C1

Plasmid

#162759

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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sgLacZs

Plasmid

#171185

Purpose

Expresses sgRNA

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgLacZ1-hU6-sgLacZ2

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-sg-tdTomato90/816

Plasmid

#179918

Purpose

Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shFAK #2

Plasmid

#198758

Purpose

conditional knockdown of FAK

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

Insert

shFAK #2 (PTK2 Human)

Expression

Mammalian

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172669

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

RNF2 pegRNA and RNF2_nick-sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTX208

Plasmid

#89268

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsYSA-gRNA01 and OsYSA-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mouse NT

Plasmid

#242421

Purpose

Expression of a non-targeting shRNA in mouse cells

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

Non-targeting shRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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1114H

Plasmid

#200640

Purpose

An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males

Depositor

Omar Akbari

Article

Smidler et al bioRxiv [Preprint]. 2023 Jul 21:2023.07.

Insert

gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.

Use

CRISPR

Expression

Insect

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pWP172

Plasmid

#198796

Purpose

Co-expression plasmid for the OLL neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pseb-3-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWP170

Plasmid

#198792

Purpose

Co-expression plasmid for the FLP, PVD neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PY48G10A.6-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWP095

Plasmid

#198789

Purpose

Co-expression plasmid for the CEP neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PF35D11.1-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWP171

Plasmid

#198795

Purpose

Co-expression plasmid for the OLL neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Peat4prom9-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pWP063

Plasmid

#198799

Purpose

Co-expression plasmid for the PHB neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Posm-10-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP026

Plasmid

#198800

Purpose

Co-expression plasmid for the PHB neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrb-6-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP097

Plasmid

#198790

Purpose

Co-expression plasmid for the CEP neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PF09E10.7-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP101

Plasmid

#198791

Purpose

Co-expression plasmid for the FLP, PVD neurons or the PHC neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pkcnl-4-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-tdTomato90/816

Plasmid

#188487

Purpose

gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pHW394 (15xUAS::GFP::let-858 3'UTR)

Plasmid

#85584

Purpose

cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

15xUAS-delta pes-10-GFP

Expression

Worm

Mutation

GFP(S65C) with three introns for expression in C.…

Promoter

15xUAS-delta pes-10-GFP

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

Plasmid

#85583

Purpose

cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

Prab-3-GAL4-SK(DBD)-VP64

Expression

Worm

Promoter

Prab-3-GAL4-SK(DBD)-VP64

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSECC-sgTom

Plasmid

#138661

Purpose

Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgTomato

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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