Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 8,583 results for: construct

Clear keywords
Showing: 381 - 400 of 8583 results

  1. LHP398 - Ub-K48R/K63R

    Plasmid
    #32167
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Expression
    Yeast
    Mutation
    K48R and K63R
    Promoter
    CUP1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  2. pSV40-GUS-P2A-Hygro

    Plasmid
    #106480
    Purpose
    Expression of GUS and Hygromycin resistance gene from SV40 promoter
    Depositor
    Alex Hewitt
    Inserts
    beta-glucuronidase
    Hygromycin B resistance
    Expression
    Mammalian and Plant
    Promoter
    SV40 promoter
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106480-plasmid-map-sequence-id-207984

    Enlarge

  3. mCherry-Paxillin-KM-N-18

    Plasmid
    #55115
    Purpose
    Localization: Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Paxillin KM
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. LHP938 - Gal1-HA-Ub

    Plasmid
    #32175
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    HA
    Expression
    Yeast
    Promoter
    Gal1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  5. LHP336 - HA-Ub-K48R

    Plasmid
    #32164
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    HA
    Expression
    Yeast
    Mutation
    K48R
    Promoter
    CUP1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  6. pBS L30 mRuby3-Rab11A

    Plasmid
    #166430
    Purpose
    Rab11A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Raphael Gaudin
    Insert
    Rab11A
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    L30 weak promoter
    Available Since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166430-plasmid-map-sequence-id-333541

    Enlarge

  7. mApple-PDHA1-N-10

    Plasmid
    #54938
    Purpose
    Localization: Mitochondria, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    PDHA1 (PDHA1 Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pGL3-Basic-OPN-600

    Plasmid
    #11997
    Depositor
    Steven Johnson
    Insert
    Osteopontin (SPP1 Human)
    Expression
    Mammalian
    Mutation
    The following construct contains the OPN promoter…
    Available Since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only

  9. mApple-Paxillin-KM-N-18

    Plasmid
    #54936
    Purpose
    Localization: Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Paxillin-KM
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pJK554

    Plasmid
    #72453
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72453-plasmid-map-sequence-id-133893

    Enlarge

  11. LHP599 - pACT2-Ub

    Plasmid
    #32170
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    Gal4 AD and HA
    Expression
    Yeast
    Promoter
    pADH1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. LPH2776 - Ub-I44A

    Plasmid
    #32163
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Expression
    Yeast
    Mutation
    I44A
    Promoter
    CUP1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  13. pET-Impact-T4gp41

    Plasmid
    #228207
    Purpose
    Expression in BL21(DE3) of T4 helicase
    Depositor
    Stephen Benkovic
    Insert
    41 (41 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228207-plasmid-map-sequence-id-453945

    Enlarge

  14. pET-Impact-T4gp59

    Plasmid
    #228206
    Purpose
    Expression in BL21(DE3) of WT T4 helicase loader
    Depositor
    Stephen Benkovic
    Insert
    59 (59 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228206-plasmid-map-sequence-id-453944

    Enlarge

  15. pTYB1-T4denV

    Plasmid
    #228216
    Purpose
    Expression in BL21(DE3) of T4 endonuclease V N-glycosylase UV repair enzyme
    Depositor
    Stephen Benkovic
    Insert
    denV
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228216-plasmid-map-sequence-id-455996

    Enlarge

  16. L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6

    Plasmid
    #219712
    Purpose
    Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-pGATA23-Bxb1-tUBQ10-GG6
    Use
    Synthetic Biology
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219712-plasmid-map-sequence-id-435863

    Enlarge

  17. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

    Plasmid
    #219709
    Purpose
    Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH:PhiC31:tUBQ1-GG4
    Use
    Synthetic Biology
    Available Since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219709-plasmid-map-sequence-id-435862

    Enlarge

  18. pcDNA-HisFlag-rUSP19ER-CRmut

    Plasmid
    #155253
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CR mutant (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Cys(545) to Arg
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155253-plasmid-map-sequence-id-311950

    Enlarge

  19. pEGFP-rUSP19CYT-CAmut

    Plasmid
    #155250
    Purpose
    for mammalian overexpression of the mutated CYT isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (Cytoplasmic isoform) CA mutant (Usp19 Rat)
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155250-plasmid-map-sequence-id-311949

    Enlarge

  20. pEGFP-rUSP19ER-CAmut

    Plasmid
    #155249
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CA mutant (Usp19 Rat)
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155249-plasmid-map-sequence-id-311947

    Enlarge

Showing: 381 - 400 of 8583 results