We narrowed to 2,723 results for: halo

1. pHaloTag-EGFP

   Plasmid

   #86629

   Purpose

   Expresses HaloTag-eGFP tandem (cytosolic)

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   HaloTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSET.HaloTag.v7.AviTag

   Plasmid

   #195486

   Purpose

   Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   HaloTag.v7.AviTag
   

   Use

   Tags

   6xHIS and AviTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSET.HaloTag.v7.AviTag

   Plasmid

   #195486

   Purpose

   Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   HaloTag.v7.AviTag
   

   Use

   Tags

   6xHIS and AviTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDule2-Mb haloTyrRS C6

   Plasmid

   #160378

   Purpose

   C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

   Depositor

   Ryan Mehl

   Article

   Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

   Insert

   C6 HaloTyrosine tRNA synthatase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   L270S, Y271L, N311G, C313T

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDule2-Mb haloTyrRS C6

   Plasmid

   #160378

   Purpose

   C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

   Depositor

   Ryan Mehl

   Article

   Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

   Insert

   C6 HaloTyrosine tRNA synthatase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   L270S, Y271L, N311G, C313T

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HA-EGFP-HaloTag2

   Plasmid

   #41742

   Depositor

   Craig Crews

   Article

   Neklesa et al Nat Chem Biol. 2011 Jul 3;7(8):538-43. d

   Insert

   Halotag 2
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HaloTag-sec61b

   Plasmid

   #186959

   Purpose

   Express sec61b connected with N-terminus HaloTag

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Sec61 (SEC61B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HaloTag-sec61b

   Plasmid

   #186959

   Purpose

   Express sec61b connected with N-terminus HaloTag

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Sec61 (SEC61B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HaloKu70 sgRNA

   Plasmid

   #207583

   Purpose

   sgRNA for the insert of the HaloTag at the endogenous loci of Ku70.

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   GAGCAGTAGCCAACATGTCA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HaloKu70 sgRNA

    Plasmid

    #207583

    Purpose

    sgRNA for the insert of the HaloTag at the endogenous loci of Ku70.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    GAGCAGTAGCCAACATGTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-CEP41_Halo

    Plasmid

    #135446

    Purpose

    Mamalian cell expression of HaloTag7 localized at the centrosome

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    CEP41-HaloTag7
    

    Use

    Tags

    CEP41

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-CEP41_Halo

    Plasmid

    #135446

    Purpose

    Mamalian cell expression of HaloTag7 localized at the centrosome

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    CEP41-HaloTag7
    

    Use

    Tags

    CEP41

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Halo_aTubulin-pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221901

    Purpose

    Expresses Halo7 fused to alpha tubulin

    Depositor

    Janelia Integrative Imaging

    Insert

    Halo7-aTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Halo_aTubulin-pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221901

    Purpose

    Expresses Halo7 fused to alpha tubulin

    Depositor

    Janelia Integrative Imaging

    Insert

    Halo7-aTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-TRPV1exCellHalo

    Plasmid

    #220307

    Purpose

    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

    Depositor

    Eric Senning

    Article

    Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

    Insert

    rTRPV1exCellHalo (Trpv1 Rat)
    

    Use

    Tags

    circularly permutated Halotag (Deo et al., Nat Ch…

    Expression

    Mammalian

    Mutation

    linker included to insert cpHaloTag (

    Promoter

    CMV promoter

    Available since

    July 30, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA5-TOM20_Halo_T2A_EGFP

    Plasmid

    #135443

    Purpose

    Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    TOM20-HaloTag7 and EGFP
    

    Use

    Tags

    TOM20

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-TRPV1exCellHalo

    Plasmid

    #220307

    Purpose

    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

    Depositor

    Eric Senning

    Article

    Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

    Insert

    rTRPV1exCellHalo (Trpv1 Rat)
    

    Use

    Tags

    circularly permutated Halotag (Deo et al., Nat Ch…

    Expression

    Mammalian

    Mutation

    linker included to insert cpHaloTag (

    Promoter

    CMV promoter

    Available since

    July 30, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pcDNA5-TOM20_Halo_T2A_EGFP

    Plasmid

    #135443

    Purpose

    Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    TOM20-HaloTag7 and EGFP
    

    Use

    Tags

    TOM20

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCoofy1-psHaloTag1a

    Plasmid

    #232940

    Purpose

    Photoswichable HaloTag: psHaloTag1a variant

    Depositor

    Claire Deo

    Article

    Walterspiel et al bioRxiv 2024.12.18.629107

    Insert

    photoswitchable HaloTag 1a variant
    

    Use

    Tags

    His6x-HRV3C

    Expression

    Bacterial

    Mutation

    E143W, A258W with linkers L1 (FAG) and L2 (P)

    Promoter

    T7

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCoofy1-psHaloTag1a

    Plasmid

    #232940

    Purpose

    Photoswichable HaloTag: psHaloTag1a variant

    Depositor

    Claire Deo

    Article

    Walterspiel et al bioRxiv 2024.12.18.629107

    Insert

    photoswitchable HaloTag 1a variant
    

    Use

    Tags

    His6x-HRV3C

    Expression

    Bacterial

    Mutation

    E143W, A258W with linkers L1 (FAG) and L2 (P)

    Promoter

    T7

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge