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pTorPE-G-GECO1.1

Plasmid

#32467

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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Lapronic/pcDNA3.1(-)

Plasmid

#140756

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1.2

Plasmid

#32468

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.2

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pTorPE-B-GECO1

Plasmid

#32469

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

B-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pTorPE-GEX-GECO1

Plasmid

#32464

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEX-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pTorPE-R-GECO1

Plasmid

#32465

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Use

Lab constructed

Tags

6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

Substitutions relative to the mApple-derived anal…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pTorPE-GEM-GECO1

Plasmid

#32463

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEM-GECO1

Use

Lab constructed

Tags

6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-ER-LAR-GECO1

Plasmid

#61244

Purpose

Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

Insert

LAR-GECO1

Use

Tags

ER-retention sequence: KDEL and ER-targeting sequ…

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: V51W/I113V/N35…

Promoter

CMV

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-mito-LAR-GECO1.2

Plasmid

#61245

Purpose

Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

Insert

LAR-GECO1.2

Use

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1.2: N45I/A47R/E1…

Promoter

CMV

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 1

Pooled Library

#168776

Purpose

Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Yeast

Species

Sars-cov-2

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 2

Pooled Library

#168777

Purpose

Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Yeast

Species

Sars-cov-2

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
pUAST-pHuji-GINKO2

Plasmid

#177117

Purpose

Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

pHuji-GINKO2

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70 promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-LUCI-GECO1

Plasmid

#113675

Purpose

Genetically-encoded ratiometric bioluminescent Ca2+ indicator based on GCaMP6s

Depositor

Robert Campbell

Article

Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

Insert

LUCI-GECO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB13089

Plasmid

#212676

Purpose

TEF promoter

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

TEF

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12706

Plasmid

#212677

Purpose

EXP promoter

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

EXP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12710

Plasmid

#212680

Purpose

Syn8 terminator

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

Syn8

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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PCfB12709

Plasmid

#212681

Purpose

SynGuo terminator

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

SynGuo

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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rEstus

Plasmid

#209767

Purpose

To express a bright and sensitive genetically encoded voltage indicator in mammalian cells.

Depositor

Stefan Heinemann

Article

Rühl et al bioRxiv 2023.

Insert

rEstus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GW

Plasmid

#27230

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pFCK(0.4)GW

Plasmid

#27229

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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