We narrowed to 1,106 results for: pFAST

1. pFAST-BAC BAF57/SMARCE1-His

   Plasmid

   #177861

   Purpose

   Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

   Depositor

   Yoshihiro Izumiya

   Article

   Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

   Insert

   SMARCE1 (SMARCE1 Human)
   

   Use

   Tags

   His tag at C-terminus with GGGGS linker

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac-Dual- GST-TEV-TBK1

   Plasmid

   #208875

   Purpose

   For expression in insect cells of TBK1

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

   Plasmid

   #216377

   Purpose

   Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

   Depositor

   William Weis

   Insert

   LDL receptor related protein 6 (LRP6 Human)
   

   Use

   Baculovirus

   Tags

   9xHis and HA signal sequence-FLAG-3C cleavage sit…

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin (LRP6)/p10 (MESD)

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac (GST-preSci site-CD45)

   Plasmid

   #177889

   Purpose

   Baculo expression of GST tag fused to human CD45 ICD

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   CD45 (aa 598-1304 only) (PTPRC Human)
   

   Use

   Tags

   TEV-GST-PreScission cut site

   Expression

   Insect

   Mutation

   L652P

   Promoter

   Polyhedrin

   Available since

   Aug. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac-GFP-dynein-2(D1091–Q4307)

   Plasmid

   #64064

   Purpose

   Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

   Depositor

   Andrew Carter

   Article

   Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

   Insert

   Cytoplasmic dynein-2 (DYNC2H1 Human)
   

   Use

   Tags

   GFP, His tag, and ZZ tag

   Expression

   Insect

   Mutation

   Deleted amino acids 1-1090, extra C-terminal vali…

   Promoter

   Polyhedrin

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1-OSF-TRIM5alpha(human)(R332P)

   Plasmid

   #79037

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(human R332P) (TRIM5 Human)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Changed Arg 332 to Pro

   Promoter

   polyhedrin

   Available since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-His-Flag-Tipin-L195A

   Plasmid

   #23120

   Depositor

   Aziz Sancar

   Article

   Kemp et al J Biol Chem. 2010 Mar 15. ():.

   Insert

   Tipin (TIPIN Human)
   

   Use

   Tags

   Flag- and His

   Expression

   Insect

   Mutation

   Changed Leucine 195 to Alanine

   Promoter

   Available since

   March 3, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pFastBac-M13-8xHis-ZZ-LTLT-HOOK3

   Plasmid

   #222287

   Purpose

   Expression of HOOK3 in insect cells for purification of untagged adapter (8xHIS-ZZtag can be cleaved off with TEV protease).

   Depositor

   Anne Straube

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Insert

   HOOK3 (HOOK3 Human)
   

   Use

   Tags

   8 His and ZZ tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST

   Plasmid

   #223764

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac HTB His-TEV-rDPP9

    Plasmid

    #217326

    Purpose

    Purification of rat DPP9 from insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

    Plasmid

    #216378

    Purpose

    Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    HA signal sequence-FLAG

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

    Plasmid

    #198037

    Purpose

    Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST-TEV-EGFP

    Expression

    Insect

    Mutation

    E696K

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac-GST-TEV-mCherry-TBK1

    Plasmid

    #198033

    Purpose

    Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST-TEV and mCherry

    Expression

    Insect

    Mutation

    E696K

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac HTB MBP-SARM1 A600G

    Plasmid

    #195764

    Purpose

    Purification of SARM1 from insect cells

    Depositor

    Hao Wu

    Insert

    SARM1 (SARM1 Human)
    

    Use

    Tags

    HIS-MBP-TEV

    Expression

    Insect

    Mutation

    A600G

    Promoter

    polyhedrin

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac-M13-6His-KIF1C_R169W-GFP

    Plasmid

    #185979

    Purpose

    Baculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    6xHis and eGFP

    Expression

    Insect

    Mutation

    R169W - mutation causing HSP (SPG58)

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac-M13-6His-KIF1C_P176L-GFP

    Plasmid

    #185978

    Purpose

    Baculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    6xHis and eGFP

    Expression

    Insect

    Mutation

    P176L - mutation causing HSP (SPG58)

    Promoter

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac1-OSF-TRIMCyp (owl monkey)

    Plasmid

    #79035

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5Cyp (owl monkey)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac(His10-Lckg2a,Y394F,Y505F)

    Plasmid

    #177894

    Purpose

    Baculo expression of His10 tag fused to human LckG2A

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Lck (LCK Human)
    

    Use

    Tags

    10xHis-TEV

    Expression

    Insect

    Mutation

    G2A,Y394F,Y505F

    Promoter

    Polyhedrin

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac His6 TEV LIC cloning vector (4B)

    Plasmid

    #30115

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac Dual PNG-FLAG PLU-HIS

    Plasmid

    #113173

    Purpose

    Baculovirus expression of PNG and PLU

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    PNG, PLU
    

    Use

    Baculovirus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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