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FUS LC-RGG1 TtoV

Plasmid

#226613

Purpose

All threonines in the LC domain to valine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 TtoV (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All threonine to valine in the LC domain

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoG

Plasmid

#226614

Purpose

All glutamines in the LC domain to glycine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoG (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All glutamine to glycine in the LC domain

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoN

Plasmid

#226615

Purpose

All glutamines in the LC domain to asparagine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoN (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All glutamine to asparagine in the LC domain

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoS

Plasmid

#226616

Purpose

All glutamines in the LC domain to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoS (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All glutamine to serine in the LC domain

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC noYQ_noYT

Plasmid

#226617

Purpose

T7S / Q160S double mutation

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC noYQ_noYT (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

T7S / Q160S double mutation in LC domain to remov…

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 wild type

Plasmid

#226596

Purpose

Wild type sequence of FUS LC-RGG1 domains

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 WT (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

wild type LC-RGG1

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4QQ->AA

Plasmid

#226600

Purpose

4 glutamine pairs to alanine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->AA (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 QQ pairs replaced with AA pairs at positions 27…

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4QQ->GG

Plasmid

#226601

Purpose

4 glutamine pairs to glycine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->GG (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 QQ pairs replaced with GG pairs at positions 27…

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4QQ->NN

Plasmid

#226602

Purpose

4 glutamine pairs to asparagine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->NN (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 glutamine pairs to asparagine pairs at position…

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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p50.mALDH1A1.CD63-GFP.wpre

Plasmid

#226770

Purpose

CD63-GFP in p52 (mAldh1a backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

CD63 (CD63 )

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet

Plasmid

#209411

Purpose

Expression of wild-type adult isoform of human SCN8A.

Depositor

Al George

Article

Vanoye et al JCI Insight. 2024 May 21;9(12):e177530.

Insert

SCN8A Variant 3, stabilized (SCN8A Human)

Expression

Mammalian

Mutation

Modifed human HSPA5 intron 4 inserted after bp237…

Promoter

CMV

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL FBM*/PBM*

Plasmid

#195221

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Expression

Mammalian

Mutation

Dual mutant for HRS>AAA / lacking DTHL

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-VcnTS-E1015A-E1021A

Plasmid

#213411

Purpose

Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnTS-E1015A-E1021A (VCL Chicken, Synthetic)

Use

Lentiviral

Mutation

Mutated vinculin glutamic acid 1015 and 1021 to a…

Promoter

CMV

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 1 HITI-2c ACTB

Plasmid

#206269

Purpose

ENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

HITI-2c donor

Use

CRISPR; Multimate/gateway entr 1

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-NQO1(Y128F)

Plasmid

#177141

Purpose

Bacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

NQO1 (NQO1 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK

Plasmid

#126651

Purpose

B6J Lrig1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

Lrig1 (Lrig1 Mouse)

Use

Cre/Lox and Mouse Targeting

Mutation

C-terminal fusion of T2A-sfGFP-iCre-ERT2

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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GST-beta2(616-951)Double

Plasmid

#100746

Purpose

Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

GST

Expression

Bacterial

Mutation

Long isoform with deletion of clathrin binding mo…

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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GST-beta2(616-951)Y815A

Plasmid

#100744

Purpose

Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

GST

Expression

Bacterial

Mutation

Long isoform with Y815A mutation

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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GST-beta2(616-951)∆CBM

Plasmid

#100745

Purpose

Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

beta2 adaptin (AP2B1 Human)

Tags

GST

Expression

Bacterial

Mutation

Long isoform with deletion of clathrin binding mo…

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5b HA Irx5 deltaHD

Plasmid

#24997

Depositor

Benoit Bruneau

Article

Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

Insert

Irx5 (Irx5 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletes entire HD (aa 112-177)

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only