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pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159843

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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GTL-y

Plasmid

#81100

Purpose

Gene Tagging vector LEU/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCitrine

Use

Cre/Lox

Tags

mCitrine

Expression

Yeast

Mutation

Mutated mCitrine for monomeric isoform (A206K L22…

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_PTK6-1/1

Plasmid

#91441

Purpose

Protein expression and purification of human SH3 domain construct PTK6-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PTK6-1/1 (PTK6 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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IRS1-B1

Plasmid

#25698

Depositor

Eric Fearon

Article

Bommer et al J Biol Chem. 2010 Jan 15. 285(3):1928-38

Insert

IRS1 (IRS1 Human)

Use

RNAi and Retroviral

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pHH0103_SH3RF1-1/4

Plasmid

#91544

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-1/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-1/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-2/4

Plasmid

#91457

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-2/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-2/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-4/4

Plasmid

#91374

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-4/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-4/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF3-2/2

Plasmid

#91356

Purpose

Protein expression and purification of human SH3 domain construct SH3RF3-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF3-2/2 (SH3RF3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-3/4

Plasmid

#91366

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-3/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-3/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF3-1/2

Plasmid

#91280

Purpose

Protein expression and purification of human SH3 domain construct SH3RF3-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF3-1/2 (SH3RF3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-T-RG

Plasmid

#102368

Purpose

Express cre dependent TVA and SADB19 RG (biscistronic) as an AAV

Depositor

Troy Margrie

Article

Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

Insert

TVA E2A-RG

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pKP4010

Plasmid

#113336

Purpose

Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Inserts

Artificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase

Tags

3X-FLAG/8X-Gly linker

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-eSpCas9

Plasmid

#126769

Purpose

Expression of increased fidelity eSpCas9 in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

eSpCas9

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3BP4-1/1

Plasmid

#91532

Purpose

Protein expression and purification of human SH3 domain construct SH3BP4-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3BP4-1/1 (SH3BP4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SORBS2-1/3

Plasmid

#91387

Purpose

Protein expression and purification of human SH3 domain construct SORBS2-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS2-1/3 (SORBS2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_VAV1-1/2

Plasmid

#91355

Purpose

Protein expression and purification of human SH3 domain construct VAV1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV1-1/2 (VAV1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Rae1-pDONR221

Plasmid

#124240

Purpose

Gateway entry clone of Drosophila Rae1 cDNA.

Depositor

Peter Vilmos

Article

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Rae1 (Rae1 Fly)

Expression

Insect

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAM-1/1

Plasmid

#91428

Purpose

Protein expression and purification of human SH3 domain construct STAM-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAM-1/1 (STAM Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PACSIN3-1/1

Plasmid

#91335

Purpose

Protein expression and purification of human SH3 domain construct PACSIN3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN3-1/1 (PACSIN3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RsACR_995-EYFP

Plasmid

#103772

Purpose

Anion channelrhodopsin from Rhodomonas salina (CCMP1319) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin RsACR_995

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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