We narrowed to 18,160 results for: Met;

1. pET19b_4CL:P7SHb + STS:P8SHb

   Plasmid

   #139793

   Purpose

   expression of biosynthetic enzymes 4CL and STS in fusion with CC

   Depositor

   Roman Jerala

   Article

   Fink et al AMB Express. 2020 May 24;10(1):97. doi:

   Inserts

   4CL:P7SHb
   STS:P8SHb
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET19b_4CL:P7S + STS:P8S

   Plasmid

   #139794

   Purpose

   expression of biosynthetic enzymes 4CL and STS in fusion with CC

   Depositor

   Roman Jerala

   Article

   Fink et al AMB Express. 2020 May 24;10(1):97. doi:

   Inserts

   4CL:P7S
   STS:P8S
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGBW-m4046191

   Plasmid

   #149115

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pGBW-m4046556

   Plasmid

   #149061

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pGBW-m4046389

   Plasmid

   #149051

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4046554

   Plasmid

   #145562

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046161

   Plasmid

   #145459

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGuide-P1-O1a (EL702)

   Plasmid

   #140040

   Purpose

   CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

   Depositor

   Johan Elf

   Article

   Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

   Insert

   barcode P1 and sgRNA lacO1 array
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET19b_4CL:P3S + STS:P4S

   Plasmid

   #139789

   Purpose

   expression of biosynthetic enzymes 4CL and STS in fusion with CC

   Depositor

   Roman Jerala

   Article

   Fink et al AMB Express. 2020 May 24;10(1):97. doi:

   Insert

   biosynthetic enzymes 4CL and STS in fusion with coiled-coil segments
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEMY07AB-PRO

    Plasmid

    #127710

    Purpose

    Level 0 destination vector for a promoter with A - B scars. Clone in with BpiI

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+) eGFP SH3BP5 G4

    Plasmid

    #129022

    Purpose

    Expresses an eGFP mRNA with the SH3BP5 G-quadruplex (G4) region in the 3' UTR

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-Nod-BFP2-TSERex

    Plasmid

    #124785

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Nod-BFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMST-1313

    Plasmid

    #118076

    Purpose

    pTEF1-cexA-tCYC1

    Depositor

    Michael Sauer

    Article

    Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

    Insert

    pTEF1-cexA-tCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET29b-clbI S178A

    Plasmid

    #114159

    Purpose

    Expresses ClbI S178A

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbI
    

    Use

    Tags

    6xHis and 6xHis-Thrombin site

    Expression

    Bacterial

    Mutation

    Mutated S178 to Ala

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-clbH-C-A2-PCP

    Plasmid

    #114157

    Purpose

    Expresses ClbH-C-A2-PCP

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbH
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Only contains the C-A2-PCP domains of ClbH

    Promoter

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET30a-RAMA

    Plasmid

    #112226

    Purpose

    Expresses RAMA domain from murine MPND

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    MPND (Mpnd Mouse)
    

    Use

    Tags

    Flag and HIs S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pS3C

    Plasmid

    #108211

    Purpose

    Expresses SsoPCNA3-CYP101A1 fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

    Insert

    SsoPCNA3-CYP101A1 fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pS1R

    Plasmid

    #108207

    Purpose

    Expresses SsoPCNA1-PdR fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

    Insert

    SsoPCNA1-PdR fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMD-SsoPCNA1

    Plasmid

    #107076

    Purpose

    S. solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA1
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    SP6, lac

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMD-SsoPCNA3

    Plasmid

    #107078

    Purpose

    S. solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA3
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    SP6, lac

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMD-SsoPCNA2

    Plasmid

    #107077

    Purpose

    S. solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA2
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    SP6, lac

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET15b-M3C

    Plasmid

    #107087

    Purpose

    M. sedula PCNA3 fused at the N-terminus of CyPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    M. sedula PCNA3 fused to CyPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET15b-S3C

    Plasmid

    #107090

    Purpose

    S. solfataricus PCNA3 fused at the N-terminus of CyPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA3 fused to CyPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET15b-S1C

    Plasmid

    #107088

    Purpose

    S. solfataricus PCNA1 fused at the N-terminus of CyPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA1 fused to CyPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET15b-YS3

    Plasmid

    #107084

    Purpose

    S. solfataricus PCNA3 fused at the C-terminus of YPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA3 fused to YPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET15b-YS1

    Plasmid

    #107082

    Purpose

    S. solfataricus PCNA1 fused at the C-terminus of YPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA1 fused to YPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET15b-M2C

    Plasmid

    #107086

    Purpose

    M. sedula PCNA2 fused at the N-terminus of CyPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    M. sedula PCNA2 fused to CyPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET15b-M1C

    Plasmid

    #107085

    Purpose

    M. sedula PCNA1 fused at the N-terminus of CyPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    M. sedula PCNA1 fused to CyPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET15b-YM3

    Plasmid

    #107081

    Purpose

    M. sedula PCNA3 fused at the C-terminus of YPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    M. sedula PCNA3 fused to YPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET15b-YM2

    Plasmid

    #107080

    Purpose

    M. sedula PCNA2 fused at the C-terminus of YPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    M. sedula PCNA2 fused to YPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET15b-YM1

    Plasmid

    #107079

    Purpose

    M. sedula PCNA1 fused at the C-terminus of YPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    M. sedula PCNA1 fused to YPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAS_gpyrG2

    Plasmid

    #90277

    Purpose

    (also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    gRNA (pyrg2)
    

    Use

    A. niger

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. P0050::rpaAD53A

    Plasmid

    #101831

    Purpose

    drives expression of rpaA D53A variant from an rpaA-independent promoter

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    RpaA D53A with p0050 promoter (+ gentamicin resistance casette)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    D53 mutated to A

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. YIp128-P30-His-pol30(K127R)

    Plasmid

    #99543

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; Leu2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8

    Plasmid

    #90285

    Purpose

    BB2 with split pyrG marker for homologous integration with linker AB (negative control)

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    pyrGtrunc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    truncated version of pyrG gene (sequence is from …

    Promoter

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8

    Plasmid

    #90286

    Purpose

    BB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    pyrGtrunc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    truncated version of pyrG gene (sequence is from …

    Promoter

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8

    Plasmid

    #90287

    Purpose

    BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    pyrGtrunc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    truncated version of pyrG gene (sequence is from …

    Promoter

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

    Plasmid

    #90288

    Purpose

    BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    pyrGtrunc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    truncated version of pyrG gene (sequence is from …

    Promoter

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Ppms028(JBEI-11601)

    Plasmid

    #87958

    Purpose

    jStack yeast assembly into plant vectors, Level 1 Intermediary Vector, p15A origin

    Depositor

    Dominique Loque

    Article

    Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pms5102(JBEI-10536)

    Plasmid

    #87969

    Purpose

    jStack yeast assembly into plant vectors

    Depositor

    Dominique Loque

    Article

    Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

    Insert

    {L_AtHsp18.2}
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge