Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 15,672 results for: bli

Clear keywords
Showing: 4001 - 4020 of 15672 results

  1. pGEX6P1-N-Strep2

    Plasmid
    #119758
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119758-plasmid-map-sequence-id-233666

    Enlarge

  2. pcDNA-mC/EBPb

    Plasmid
    #49198
    Purpose
    expression of mouse C/EBPbeta in mammalian cells
    Depositor
    Jed Friedman
    Insert
    C/EBPbeta (Cebpb Mouse)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    49198-plasmid-map-sequence-id-265902

    Enlarge

  4. pHA AKT2 T309A/S474A

    Plasmid
    #60128
    Purpose
    expresses a mutant AKT2
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B (AKT2 Human)
    Use
    Retroviral
    Tags
    HA
    Mutation
    Serine 474 and Threonine 309 changed to alanines
    Promoter
    CMV
    Available Since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. mu-FLT3Lv1-pTRIP

    Plasmid
    #163519
    Purpose
    Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells
    Depositor
    Thomas Weber
    Insert
    murine-FLT3L variant 1 (Flt3l Mouse)
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163519-plasmid-map-sequence-id-321571

    Enlarge

  7. pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-mKate2

    Plasmid
    #51092
    Purpose
    Forebrain principal neuron expression of oChIEF kinetic variant E163A/T199C with physically uncoupled mKate2 red fluorophore
    Depositor
    Jonathan Ting
    Insert
    oChIEF(E163A/T199C)
    Use
    AAV
    Tags
    P2A-mKate2
    Expression
    Mammalian
    Mutation
    E163A/T199C
    Promoter
    CaMKIIa
    Available Since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51092-plasmid-map-sequence-id-79306

    Enlarge

  8. pHD-DsRed-attP-w+

    Plasmid
    #80898
    Purpose
    Vector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    GMR-white
    Use
    High copy, amp resistance
    Promoter
    3xP3 and GMR::hsp70
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    80898-plasmid-map-sequence-id-172486

    Enlarge

  9. OptoEphB2-mCherry

    Plasmid
    #92042
    Purpose
    Expresses OptoEphB2 labeled with mCherry
    Depositor
    Ji Yu
    Insert
    EPHB2 (EPHB2, CRY2 Human, Mustard Weed)
    Tags
    mCherry
    Expression
    Mammalian
    Available Since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    92042-plasmid-map-sequence-id-182371

    Enlarge

  10. Human-Dysferlin-Original

    Plasmid
    #60216
    Purpose
    Human dysferlin (accession #NM_003494) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Gateway entry clone
    Available Since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    60216-plasmid-map-sequence-id-96330

    Enlarge

  11. pLenti spCas9 T2A TagRFP-T P2A puro

    Plasmid
    #122200
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9
    TagRFP-T
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Available Since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    122200-plasmid-map-sequence-id-236123

    Enlarge

  12. HMGCS2

    Plasmid
    #38984
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HMGCS2 (HMGCS2 Human)
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pGEX6P1-N-FLAG

    Plasmid
    #119759
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    FLAG and GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119759-plasmid-map-sequence-id-233663

    Enlarge

  15. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Bob Goldstein
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    70684-plasmid-map-sequence-id-306412

    Enlarge

  16. pEG302-SunTagng-22aa

    Plasmid
    #115345
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome
    Depositor
    Steven Jacobsen
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    115345-plasmid-map-sequence-id-222916

    Enlarge

  17. pQE80L-IL2 C125S

    Plasmid
    #104348
    Purpose
    Expresses human IL-2 (C125S)
    Depositor
    Alexander McLellan
    Insert
    Human IL-2 (C125S) (IL2 Human)
    Tags
    MRGSHHHHHGS-tag
    Expression
    Bacterial
    Mutation
    C125S mutation (TGT -> TCC)
    Promoter
    T5
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104348-plasmid-map-sequence-id-201216

    Enlarge

  18. mu-IFNg-pCIneo

    Plasmid
    #163517
    Purpose
    Expresses murine IFN gamma in mammalian cells
    Depositor
    Thomas Weber
    Insert
    murine-IFN gamma (Ifng Mouse)
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163517-plasmid-map-sequence-id-321578

    Enlarge

  20. BACH1

    Plasmid
    #38837
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BACH1 (Bach1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 4001 - 4020 of 15672 results