We narrowed to 47,344 results for: cha;
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Plasmid#53707PurposeLuciferase reporter assay for CIP2A with point mutation on miR-375 binding siteDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseMutationMutation on miR-375 binding site B (refer to cita…Available SinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A-miR-375-mutA
Plasmid#53706PurposeLuciferase reporter assay for CIP2A with point mutation on miR-375 binding siteDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseMutationMutation on miR-375 binding site A (refer to cita…Available SinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-active WRN
Plasmid#125788Purposeconstitutive, ectopic expression of human active WRNDepositorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-WRN K577M
Plasmid#125790Purposeconstitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)DepositorAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-WRN E84A
Plasmid#125789Purposeconstitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)DepositorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-WRN E84A K577M
Plasmid#125791Purposeconstitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRNDepositorInsertmyc-WRN E84A/K577M (WRN Human)
Tags5x mycExpressionMammalianMutationE84A/K577M (exonuclease/helicase-dead)Available SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD051-sgMLH1-1
Plasmid#125776Purposeconstitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9DepositorInsertsgMLH1-1 (MLH1 Human)
UseCRISPRAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD051-sgMLH1-2
Plasmid#125777Purposeconstitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9DepositorInsertsgMLH1-2 (MLH1 Human)
UseCRISPRAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28 His-MBP-TEV-BAP-Human BCKDK
Plasmid#232116PurposeFor bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.DepositorInsertBCKDK (BCKDK Human)
Tags6 x His, Biotin acceptor peptide (BAP), Maltose-B…ExpressionBacterialPromoterT7 PromoterAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003-sgWRN-EIJ
Plasmid#125774Purposeconstitutive expression of a guide RNA targeting an exon-intron junction of human WRNDepositorInsertsgWRN-EIJ (WRN Human)
UseCRISPRAvailable SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-shPSMD2
Plasmid#125779Purposeconstitutive expression of a short-hairpin RNA targeting human PSMD2 (RNAi positive control)DepositorInsertshPSMD2 (PSMD2 Human)
UseRNAiAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003-sgPOLR2D
Plasmid#125769Purposeconstitutive expression of a guide RNA targeting human POLR2D (CRISPR positive control)DepositorInsertsgPOLR2D (POLR2D Human)
UseCRISPRAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJTB126
Plasmid#248910Purposeexpresses the yeast analog-sensitive Smk1-Q120A and Ssp2 fused to glutathione-S-transferase in bacteriaDepositorInsertsSMK1 Mitogen-activated Protein Kinase -analog-sensitive form (SMK1 Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes from S288C form, Budding Yeast)
Ssp2 Activator of the Smk1 MAPK (SSP2 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form)
Tagsglutathione-S-transferaseExpressionBacterialMutationcodon 120 has been changed from Q to A (Smk1-Q120…PromoterT7Available SinceJan. 16, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLX304-KDM6A-M1190T/E1335D
Plasmid#203581PurposeExpresses mutant version of KDM6A with increased oxygen affinity in mammalian cells.DepositorInsertKDM6A (KDM6A Human)
UseLentiviralTagsHA-taggedExpressionMammalianMutationChanged Methionine 1190 Threonine and Glutamic Ac…PromoterCMVAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP-TEV-Rat BCKDK-His
Plasmid#232119PurposeFor bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.DepositorInsertBCKDK (Bckdk Rat)
Tags6 x His, Maltose-Binding Protein (MBP), and Tev S…ExpressionBacterialPromoterT7 PromoterAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-2P-N317P
Plasmid#196378PurposeMammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertS-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A-stop-mut-miR-375-mutA-E
Plasmid#53714PurposeLuciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutationsDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseMutationMutation on the stop codon of firefly luciferase …Available SinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPS04
Plasmid#169472PurposeBroad-host-range allelic exchange vectorDepositorTypeEmpty backboneUseAllelic exchange vectorAvailable SinceJune 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVQ CMV NanoV1Mutant-2a-EGFP ferritin
Plasmid#79650PurposeExpresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion proteinDepositorInsertsUseAdenoviralExpressionMammalianMutationisoleucine 679 changed to lysinePromoterCMVAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28 E2 LBD-Tev-E1a-His
Plasmid#232117PurposeFor bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.DepositorInsertDBT (DBT Human)
Tags6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…ExpressionBacterialPromoterT7 PromoterAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only