We narrowed to 5,343 results for: 290

1. pLenti-Blast-sgNT

   Plasmid

   #138678

   Purpose

   Expresses a Non-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNT
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. L309-myc-seipin-WT

   Plasmid

   #64061

   Purpose

   myc-tagged murine seipin (wild type); lentiviral construct

   Depositor

   Weiping Han

   Article

   Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

   Insert

   bscl2 (Bscl2 Mouse)
   

   Use

   Lentiviral

   Tags

   myc epitope

   Expression

   Mammalian

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

3. Plxnb1(L)-Fc-His

   Plasmid

   #72126

   Purpose

   Expresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxnb1 (Plxnb1 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBTK569

   Plasmid

   #110614

   Purpose

   BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   E2-Crimson
   

   Expression

   Bacterial

   Mutation

   V32M (see comments below)

   Available Since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sema3g.a(L)-Fc-His

   Plasmid

   #72151

   Purpose

   Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3g.a (Sema3g Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR4-huNFATc1nuc

   Plasmid

   #23988

   Depositor

   Jerry Crabtree

   Article

   Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

   Insert

   NFATc1 (NFATC1 Human)
   

   Use

   Cloning vector

   Tags

   EE tag

   Mutation

   S172A S175A S176A S178A S179A S181A S184A S187A S…

   Available Since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBacDual with LFn + Needle

   Plasmid

   #84868

   Purpose

   Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Needle
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSECC-sgTom

   Plasmid

   #138661

   Purpose

   Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgTomato
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKS18

   Plasmid

   #24629

   Depositor

   Jay Keasling

   Article

   Steen et al Nature. 2010 Jan 28;463(7280):559-62.

   Insert

   mFar1 (Far1 Mouse)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIneo-V5-SBP-HseIF4AII_AC

    Plasmid

    #148439

    Purpose

    Mammalian Expression of HseIF4AII

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HseIF4AII (EIF4A2 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBTK614

    Plasmid

    #110608

    Purpose

    BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBTK601

    Plasmid

    #110607

    Purpose

    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-Hygro-sgTom

    Plasmid

    #138698

    Purpose

    Expresses a tomato-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgTomato
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-SARS-CoV-2 SL1 alone-Luciferase

    Plasmid

    #191482

    Purpose

    Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

    Depositor

    Hao Wu

    Article

    Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

    Insert

    SARS-CoV-2 5' UTR SL1 alone
    

    Use

    Lentiviral and Luciferase

    Mutation

    Contains only the stem-loop1 region of SARS-CoV-2…

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sema3g.a(L)-AP-His

    Plasmid

    #72025

    Purpose

    Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3g.a (Sema3g Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Mutation

    D10A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2Apuro-TRE-synPCB2.0

    Plasmid

    #139481

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    

    Use

    Tol2 transposon donor vector

    Expression

    Mammalian

    Promoter

    Tet responsive element

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSUPERpuro-BglII-Hs4E-T_AC

    Plasmid

    #148445

    Purpose

    Mammalian Expression of Hs4E-T-shRNA

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    Hs4E-T-shRNA (EIF4ENIF1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. L309-myc-seipin-CT

    Plasmid

    #64063

    Purpose

    myc-tagged murine seipin (cytosolic C-terminus); lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

    Insert

    bscl2 (Bscl2 Mouse)
    

    Use

    Lentiviral

    Tags

    myc epitope

    Expression

    Mammalian

    Mutation

    Contains Arg326 to Ser443

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

    Plasmid

    #124859

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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