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NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

Plasmid

#89994

Purpose

Donor template for generation of NGN3-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)

Use

CRISPR

Promoter

none

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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CT-Nano-lantern(ATP1)/pENTR1A

Plasmid

#51991

Purpose

Gateway entry vector for Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Use

Entry vector

Tags

chloroplast transit peptid

Mutation

S257G in Rluc8

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pEIIIA

Plasmid

#14059

Depositor

Richard Hynes

Article

Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

Insert

Rat Fibronectin EIIIA (Fn1 Rat)

Expression

Mammalian

Mutation

EcoRI linkers added to TaqI to HinfI fragment

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
nPEX-LV

Plasmid

#12119

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Mutation

noncatalytic fragment of MMP2 amplified via PCR, …

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pJK413

Plasmid

#155372

Purpose

dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z18

Plasmid

#120061

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(ATP1)/pRSETB

Plasmid

#51988

Purpose

bacterial expression of a Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pLX302-NIPP1-V5 puro

Plasmid

#87775

Purpose

Expresses V5 tagged human NIPP1

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

NIPP1 (PPP1R8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_63/pcDNA3

Plasmid

#51986

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-4GS

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRc2_BirA

Plasmid

#136521

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA

Use

Entry vector for gateway cloning

Mutation

R118G (highly promiscuous form)

Available Since

June 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJK429

Plasmid

#155376

Purpose

PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TT miRc2 3xFLAG

Plasmid

#83274

Purpose

Empty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAG

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Type

Empty backbone

Use

Entry vector for gateway cloning

Tags

3xFLAG

Promoter

TRE tight

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-Hemmar-FLP(D)

Plasmid

#119895

Purpose

FLP(D) with a hsp70 promoter

Depositor

Rita Sousa-Nunes

Article

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Insert

FLP(D)

Use

Drosophila expression

Mutation

mutated form of FLP, which at position 5 contains…

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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SEPLuc

Plasmid

#141102

Purpose

bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc

Use

Lentiviral

Promoter

EF1α

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcFN-C (chick fibronectin)

Plasmid

#14063

Depositor

Richard Hynes

Article

Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

Insert

Chicken Fibronectin (FN1 Chicken)

Expression

Mammalian

Mutation

DraI - EcoRI fragment inserted in HincII-EcoRI si…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT3

Plasmid

#79500

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3 (WNT3 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z04

Plasmid

#120047

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P30

Plasmid

#120041

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

VSV-G

Use

Pcr template

Tags

VSV-G

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(ATP1)/pENTR1A

Plasmid

#51993

Purpose

Gateway entry vector for Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Use

Entry vector

Mutation

S257G in Rluc8

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(ATP0)/pRSETB

Plasmid

#51989

Purpose

bacterial expression of a Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP0

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only