We narrowed to 25,030 results for: Nes

1. pCMV-erHRP(N175S mutant)

   Plasmid

   #79909

   Purpose

   erHRP(N175S mutant) pCMV backbone

   Depositor

   Joshua Sanes

   Article

   Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

   Insert

   erHRP(N175S mutant)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   N175S mutation

   Promoter

   CMV

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pbGH

   Plasmid

   #118061

   Purpose

   GBPart - Bovine growth hormone terminator

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   bgH_poly(A)
   

   Use

   Synthetic Biology; Gbpart

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. RalGDS-YPet

   Plasmid

   #134929

   Purpose

   Fluorescently tagged affinity reagent

   Depositor

   Klaus Hahn

   Article

   O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

   Insert

   RaIGDS RBD, YPet
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Orange Nano-lantern(Ca2+)/pcDNA3

   Plasmid

   #71346

   Purpose

   Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Orange Nano-lantern(Ca2+)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pVAX_CBI_AcrIIA4

   Plasmid

   #136662

   Purpose

   Expresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

   Depositor

   Yannick Doyon

   Article

   Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

   Insert

   AcrIIA4
   

   Use

   Synthetic Biology

   Tags

   No tag, No NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-NFAT5

   Plasmid

   #79506

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   NFAT5 (NFAT5 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDragon-empty

   Plasmid

   #155018

   Purpose

   Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

   Depositor

   Alberto Rosello-Diez

   Article

   Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. EE-BE4max

   Plasmid

   #138158

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(EE)-nSpCas9-UGI-UGI
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 R126E + R132E; within Cas9 D10A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEYFP-Pds1(434)

   Plasmid

   #39847

   Depositor

   Jonathon Pines

   Article

   Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

   Insert

   Pds1 (PTTG1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJSK418

    Plasmid

    #205131

    Purpose

    Expression of GFP-2xFYVE in Dictyostelium

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    2xFYVE (from human Hrs) (HGS Synthetic)
    

    Use

    Dictyostelium expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

    Plasmid

    #124861

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. KAP3-FL

    Plasmid

    #51413

    Purpose

    expression of EGFP-tagged full length human KAP3B in mammalian cells

    Depositor

    Trina Schroer

    Article

    Brown et al Traffic. 2005 Dec;6(12):1114-24.

    Insert

    Kinesin-associated protein 3 (KIFAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pNLuc

    Plasmid

    #118058

    Purpose

    GBPart - NLuc Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Nano Luciferase
    

    Use

    Luciferase and Synthetic Biology; Gbpart

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVAX_CBI_AcrIIA2

    Plasmid

    #136661

    Purpose

    Expresses human codon-optimized AcrIIA2 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

    Depositor

    Yannick Doyon

    Article

    Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

    Insert

    AcrIIA2
    

    Use

    Synthetic Biology

    Tags

    No tag, No NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHygromycin (GB0211)

    Plasmid

    #68204

    Purpose

    Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Hygromycin resistance gene coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGE-attBTT-loxP-DsRed (pTL780)

    Plasmid

    #115160

    Purpose

    Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    attB-TT loxP 3xP3-DsRed
    

    Use

    Cre/Lox

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. hCMV-IE1:GLuc:bgH

    Plasmid

    #135294

    Purpose

    Transcriptional unit for constitutive expression of GLuc in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:GLuc:bGH
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLuciferase (GB0096)

    Plasmid

    #68201

    Purpose

    Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luciferase coding region
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Orange Nano-lantern/pcDNA3

    Plasmid

    #67661

    Purpose

    orange luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

20. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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