We narrowed to 8,106 results for: Dos;

1. plenti hSyn PGK1-HALO

   Plasmid

   #220910

   Purpose

   Neuronal specific expression of the fusion construct PGK1-HALO

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1-HALO (PGK1 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC841 - pAAV minP secNLuc

   Plasmid

   #82498

   Purpose

   Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

   Depositor

   Brandon Harvey

   Article

   Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

   Insert

   secNLuc
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   c-Fos minimal promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_PDCD6_EF-hand

   Plasmid

   #110009

   Purpose

   Protein expression and purification of PDCD6_EF-hand

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PDCD6_EF-hand (PDCD6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. tdKatushka2-VASP-5

   Plasmid

   #56049

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 586, Emission: 632

   Depositor

   Michael Davidson

   Article

   Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

   Insert

   VASP
   

   Use

   Tags

   tdKatushka2

   Expression

   Mammalian

   Mutation

   A209T in VASP

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. PXPR007 sgGFP-1

   Plasmid

   #74960

   Purpose

   Cas9 + sgGFP-1 with blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

   Plasmid

   #78509

   Purpose

   Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   CD45R0Ex (PTPRC Human)
   

   Use

   Tags

   His10 and SNAPf

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mVenusN-Cavin3

   Plasmid

   #108889

   Purpose

   Expression of Cavin3 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   Cavin3 (altered codon usage) (CAVIN3 Synthetic, Human)
   

   Use

   Tags

   mVenus1-155

   Expression

   Mammalian

   Mutation

   altered codon usage

   Promoter

   Available since

   March 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-EGFP-C1-HsDCP2-E148Q_U

   Plasmid

   #147650

   Purpose

   Mammalian Expression of HsDCP2-E148Q

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsDCP2-E148Q (DCP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations compared to the sequence giv…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDG330

   Plasmid

   #100898

   Purpose

   SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMP-EZH1_1

    Plasmid

    #36359

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EZH1 (EZH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pSMP-G9A_1

    Plasmid

    #36395

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    G9A (EHMT2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pHH0103_PIKFYVE_DEP

    Plasmid

    #109789

    Purpose

    Protein expression and purification of PIKFYVE_DEP

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PIKFYVE_DEP (PIKFYVE Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-CenpB 1-166

    Plasmid

    #108475

    Purpose

    expression of GFP-CenpB 1-166

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    CenpB 1-166 AA (CENPB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    encodes only the DNA-binding domain of CENP-B

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_RAD51_Rad51

    Plasmid

    #110031

    Purpose

    Protein expression and purification of RAD51_Rad51

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RAD51_Rad51 (RAD51 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192682

    Purpose

    Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTXB1-H2B(1-114)_wt

    Plasmid

    #118252

    Purpose

    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    xlH2B(1-114)
    

    Use

    Tags

    Mxe-GyrA intein fused to chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSMP-Dot1L_1

    Plasmid

    #36339

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Dot1L (DOT1L Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pMpGWB122

    Plasmid

    #68576

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-PH-SWAP70

    Plasmid

    #84582

    Purpose

    Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

    Insert

    SWAP70 residues 209-306 (SWAP70 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Pleckstrin-homology domain, residues 209-306

    Promoter

    CMV

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only