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pEBB-BT

Plasmid

#36295

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Type

Empty backbone

Tags

BT-tag (peptide sequence from P.freudenreichii tr…

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 18, 2012

Availability

Academic Institutions and Nonprofits only

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pSB3K3-IC15

Plasmid

#165408

Purpose

Bacteria gene circuit on medium copy backbone.

Depositor

Xiaojun Tian

Article

Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

Insert

IC15 gene circuit

Expression

Bacterial

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pX165-xCas9

Plasmid

#140568

Purpose

Expresses xCas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

A262T R324L S409I E480K E543D M694I E1219V

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-19a-20-19b

Plasmid

#24827

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

mir19a-mir20-mir19b

Use

Retroviral

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pSYC-102

Plasmid

#74790

Purpose

pCS4+-NLS-EGFP-T2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

NLS-EGFP-T2A-mCherry-CAAX

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-APIC-LexAGAD

Plasmid

#112807

Purpose

gateway cloning destination vector to express enhancer driven LexAGAD in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pXM03

Plasmid

#121150

Purpose

This plamid was used for biosynthesis of Prenylated 4‑Hydroxybenzoate in E.coli

Depositor

Minjuan Xu

Article

He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

Insert

XimB, IDS2

Use

Synthetic Biology

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAJ50

Plasmid

#14918

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

enhanced monomeric red fluorescent protein

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAJ08

Plasmid

#14912

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis actin promoter

Tags

GFP

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pIMP1-Padc

Plasmid

#90486

Purpose

Plasmid with adc(CA_P0165) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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C-12-BE

Plasmid

#135040

Purpose

C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjCDA1L2_1

Use

CRISPR

Expression

Mammalian

Promoter

U6 with EF-1alpha intron

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCas9–mKate2ps–EgRNA

Plasmid

#62716

Purpose

Empty; no U6 gRNA cassette

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus Insulin 2

Plasmid

#25786

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

Insert

Insulin 2

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
N-8-BE

Plasmid

#135023

Purpose

C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_1

Use

CRISPR

Expression

Mammalian

Promoter

U6 with EF-1alpha intron

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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ptreCas9-mKate2ps-T1gRNA

Plasmid

#62715

Purpose

Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET21b - S. thermophilus DltB

Plasmid

#132607

Purpose

expresses S. thermophilus DltB in E. coli

Depositor

Wenqing Xu

Article

Ma et al Nature. 2018 Oct;562(7726):286-290. doi:

Insert

Dltb

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSYC-104

Plasmid

#74792

Purpose

pCS4+-NLS-EGFP-F2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

NLS-EGFP-F2A-mCherry-CAAX

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAJ20

Plasmid

#14919

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

Strongyloides stercoralis Ribosomal protein small subunit

Tags

GFP(S65C)

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST-APIC-Flp1

Plasmid

#112806

Purpose

gateway cloning destination vector to express enhancer driven Flip1 in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEMBL-Si-EGFP

Plasmid

#20692

Depositor

Tom Misteli

Article

Mejat et al J Cell Biol. 2009 Jan 12. 184(1):31-44.

Insert

U6-siEGFP

Use

Adeno associated virus

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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