We narrowed to 5,504 results for: crispr cas9 grna plasmid

Showing: 4061 - 4080 of 5504 results

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pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)

Plasmid

#223148

Purpose

Expression of truncated MECP2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1300-AAV-EFSNC-dCjCas9-MBD2(176-231)

Plasmid

#223145

Purpose

Expression of truncated MBD2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD2 (MBD2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 176-231

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)

Plasmid

#223144

Purpose

Expression of truncated MBD1 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD1 (MBD1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 529-592

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1306-AAV-EFSNC-dSaCas9-HP1aNH(115-177)

Plasmid

#223151

Purpose

Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 115-177

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)

Plasmid

#223141

Purpose

Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 115-177

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1309-AAV-EFSNC-dSaCas9-MBD1(529-592)

Plasmid

#223154

Purpose

Expression of truncated MBD1 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD1 (MBD1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 529-592

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1313-AAV-EFSNC-dSaCas9-MECP2(204-310)

Plasmid

#223158

Purpose

Expression of truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

Plasmid

#223155

Purpose

Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD2 (MBD2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 176-231

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pspCas9-3exo-Tetra-com-CLCN5-sp-g1

Plasmid

#176236

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176237

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176238

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pACT1:Cas9-GFP, U6:sgTK

Plasmid

#122852

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgTK

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pACT1:Cas9-GFP, U6:sgUPRT

Plasmid

#122853

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgUPRT

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9

Plasmid

#135964

Purpose

Expresses SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Sa-SauriCas9

Plasmid

#135967

Purpose

Expresses Sa-SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-DIO-SaCas9-U6-sgGabbr1

Plasmid

#240044

Purpose

Knockdown expression of Gabbr1

Depositor

Huaibin Cai

Article

Dong et al Nat Commun. 2025 Mar 19;16(1):2710. doi:

Insert

gamma-aminobutyric acid type B receptor subunit 1 (Gabbr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-en31FnCas9ABEmax8.17d

Plasmid

#201956

Purpose

Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dSpCas9

Plasmid

#201953

Purpose

Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dSpCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A on SpCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ABE-NT-sgRNA

Plasmid

#112734

Purpose

AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE-NT-sgRNA

Use

AAV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-sgCTRL

Plasmid

#209750

Purpose

Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

Cas9

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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