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pSPgRNA_BLK_E2-4

Plasmid

#124477

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-4_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_E3-3

Plasmid

#124484

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E3-3_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 CortW22A mCherry

Plasmid

#187268

Purpose

Lenticiral expression of mCherry-Cortactin W22A

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

mcherry

Mutation

W22A

Promoter

5' LTR

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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BMP10 N67Q N131Q D4G (S2-2)

Plasmid

#184622

Purpose

for transfection into S2 cells and expression of BMP10 protein

Depositor

Timothy Springer

Article

Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

Insert

BMP10 (BMP10 Human)

Tags

8x His - streptavidin binding peptide

Expression

Insect

Mutation

N67Q, N131Q, ARIRR(312-316) replaced with G

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG1856_A

Plasmid

#145905

Purpose

Insect Expression of CG1856-3UTR

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jan . 15(1):21-32.

Insert

CG1856-3UTR (ttk Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSP72-RBM8A-3'UTR AluY-Sz mut 2598-4066

Plasmid

#175586

Purpose

in vitro transcription of mut RBM8A 3'UTR

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A-3'UTR AluY-Sz mut 2598-4066

Use

In vitro transcription

Promoter

T7

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHA853 - mec-4p::Fynempty

Plasmid

#139210

Purpose

Control vector for Fyn kinase expression in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA850 - osm-10p::FynY531F

Plasmid

#139207

Purpose

Express activated Fyn kinase in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

Y531F

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA851 - osm-10p::Fynempty

Plasmid

#139208

Purpose

Control vector for Fyn kinase expression in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_withR

Plasmid

#139128

Purpose

express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Expression

Bacterial

Mutation

add arginines

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_nocharge_wR

Plasmid

#139119

Purpose

express hnRNPA2 LC with no charged residues with R added back

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

remove all K, most D

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_charge

Plasmid

#139126

Purpose

express FUS LC with an hnRNPA2 LC-like charge patterning

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Expression

Bacterial

Mutation

Add R, K, D to make FUS LC have similar charge pa…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.2

Plasmid

#149431

Purpose

miniTdg gene, SUMOylation mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

K341A SUMOylation mutant, SNPs of Tdg gene in OLA…

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_04

Plasmid

#158105

Purpose

CaV1.3 5'UTR luciferase reporter

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR of CACNA1D (uc003dgu.5)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_03

Plasmid

#158104

Purpose

CaV1.2 5'UTR luciferase reporter

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR of CACNA1C (uc009zdu.1)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Sph ELL2

Plasmid

#127270

Purpose

For in vitro translation of human WT ELL2 with internal HA tag

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

WT Mets and HA tag at SphI site (8th Met)

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met138ILeu ELL2

Plasmid

#127260

Purpose

For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met138 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met138 to Ileu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met186ILeu ELL2

Plasmid

#127261

Purpose

For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met186 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met186 to Ileu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met138ILeu, Met186ILeu ELL2

Plasmid

#127265

Purpose

For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Two Mets (138,186) to ILeu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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