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pHAGE-eGFP-TAX1BP1 CC1D

Plasmid

#175774

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC1D

Depositor

Wade Harper

Publication

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Mutation

CC1D

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pONL-N1_PTS2

Plasmid

#65677

Purpose

Expresses PTS2-ONL in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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mKO-Tubulin-6

Plasmid

#57850

Purpose

Localization: Microtubules, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Publication

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Tubulin (TUBA1B Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEX4T3-GST-HA-Ub-K48

Plasmid

#187590

Purpose

HA tagged ubiquitin with all the Ks mutated into R except K48.

Depositor

Robert Sobol

Publication

Fang et al Nucleic Acids Res. 2026 Apr 13;54(7):gka

Insert

Ubiquitin

Tags

GST

Expression

Bacterial

Mutation

K6R/K11R/K27R/K29R/K63R

Promoter

Tac

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-HA-Ub-KO

Plasmid

#187591

Purpose

HA tagged ubiquitin with all the Ks mutated into R.

Depositor

Robert Sobol

Publication

Fang et al Nucleic Acids Res. 2026 Apr 13;54(7):gka

Insert

Ubiquitin

Tags

GST

Expression

Bacterial

Mutation

K6R/K11R/K27R/K29R/K48R/K63R

Promoter

Tac

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-VPS4A E228Q

Plasmid

#246736

Purpose

Mammalian expression of human VPS4A E228Q (dominant negative form) with a HA tag on its N-terminus

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

VPS4A E228Q (DN) (VPS4A Human)

Tags

HA

Expression

Mammalian

Mutation

E228Q

Promoter

CMV

Available since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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pLD1112 TRE-mCherry-SKL-22Xm6A MS2

Plasmid

#235128

Purpose

mCherry reporter plasmid for live cell visualization of RNA m6A modification

Depositor

Qin Peng

Publication

Li et al Sci Adv. 2024 Nov 15;10(46):eadp5689. do

Insert

TRE-mCherry-SKL-22Xm6A MS2

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Dre-nls (JDW 1131)

Plasmid

#229809

Purpose

A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DRE recombinase-nls

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-DEST (JDW 1186)

Plasmid

#229820

Purpose

An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

AAV

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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N-terminal Halo-Ku70

Plasmid

#207547

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

Depositor

Jens Schmidt

Publication

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with flanked by human Ku70 locus sequences

Tags

HaloTag

Expression

Mammalian

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_GNB1L_dTAG

Plasmid

#224378

Purpose

Generates GNB1L dTAG C terminal knock-in cell lines

Depositor

Junjie Chen

Publication

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Tags

dTAG

Expression

Bacterial

Mutation

Synonymous mutation

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L

Plasmid

#224381

Purpose

Lenti plasmid for generating GNB1L expressing stable cell lines

Depositor

Junjie Chen

Publication

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-EFS (EF1a core) (JDW 1319)

Plasmid

#224474

Purpose

Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS promoter

Expression

Mammalian

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

Plasmid

#224506

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-oMDC-LexOP-c-Fos

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV(T9)-DERA-sgresis

Plasmid

#222622

Purpose

Lentiviral vector that expresses sgRNA resistant DERA in mammalian cells

Depositor

Daniel Durocher

Publication

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

DERA (DERA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEB018

Plasmid

#215568

Purpose

Fuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locus

Depositor

Robin Ohm

Insert

3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator

Use

Fungal homologous recombination plasmid

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-MiniR

Plasmid

#208328

Purpose

MiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.

Depositor

Kimberly Beatty

Publication

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

H2B-MiniR

Tags

MiniR

Expression

Mammalian

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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TREER-Actin

Plasmid

#208330

Purpose

TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

Depositor

Kimberly Beatty

Publication

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER-Actin

Tags

TREER

Expression

Mammalian

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TOMM20-PuRRRE

Plasmid

#208329

Purpose

PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

Depositor

Kimberly Beatty

Publication

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TOMM20-PuRRRE

Tags

PuRRRE

Expression

Mammalian

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-mCherry-WPRE

Plasmid

#202620

Purpose

Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Publication

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

mCherry

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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