We narrowed to 9,801 results for: otos

1. pLenti-Cdc42-2G

   Plasmid

   #68813

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Lentiviral

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1-Nst-CAG-DEST

   Plasmid

   #80489

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CIB81-pmGFP

   Plasmid

   #75366

   Purpose

   Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CIB1 (CIB1 Mustard Weed)
   

   Use

   Tags

   prenylated EGFP

   Expression

   Mammalian

   Mutation

   residues 1-81 of CIB1

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9n(BB)-2A-Puro (PX462)

   Plasmid

   #48141

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209653

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95A.A119L

   Promoter

   CAG

   Available since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-HLA-A2_GFP

   Plasmid

   #85161

   Purpose

   HLA.A2_GFP fusion with 2 glycines in between

   Depositor

   Sébastien Walchli, Tone Fredsvik Gregers

   Article

   Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

   Insert

   HLA.A2 (HLA-A Human)
   

   Use

   Gateway entry vector

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Green Lindoblum

   Plasmid

   #163113

   Purpose

   Expresses biosensor for lactate in mammalian cells

   Depositor

   Takashi Tsuboi

   Article

   Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

   Insert

   Green Lindolum
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-calpainsensor

   Plasmid

   #36182

   Purpose

   detection of activity of ubiquitous calpains

   Depositor

   Isabelle Richard

   Article

   Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

   Insert

   alpha-Fodrin cleavage site (Sptan1 Mouse)
   

   Use

   Tags

   eCFP and eYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-Aka-P2A-GFP

   Plasmid

   #120867

   Purpose

   Piggybac transposon plasmid for live animal optical imaging

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   Akaluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   insert Akaluc and a P2A site

   Promoter

   CAG Promoter

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

    Plasmid

    #139484

    Purpose

    PCR template for mEGFP gene knock-in donor vector

    Depositor

    Kazuhiro Aoki

    Article

    Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

    Insert

    mEGFP-loxP-P2A-dTKneo-pA-loxP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2p2A--3mnx1:mTagBFP-NS-MCS

    Plasmid

    #78250

    Purpose

    Can be used to subclone in gene of interest into blue motor neuron expression vector.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Inserts

    -3mnx1 (mnx1 Zebrafish)
    -mTagBFP-NS-MCS
    

    Use

    Zebrafish motor neuron expression

    Tags

    Expression

    Mutation

    Promoter

    -3mnx1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-FLIM-AKAR

    Plasmid

    #63058

    Purpose

    Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDF0352 HvsCas7-11 with C-term Nes mammalian expression

    Plasmid

    #172516

    Purpose

    Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    C-term Nes HvsCas7-11 mammalian expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-25xUPRE-minP-EGFP

    Plasmid

    #159668

    Purpose

    EGFP reporter plasmid containing 25 tandem repeats of the the unfolded protein response element (UPRE)

    Depositor

    Seiichi Oyadomari

    Article

    Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

    Insert

    25xUPRE-minP-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRE-DIO-eYFP

    Plasmid

    #117383

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV1

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TB:5xNF- κβ_RE:MiniP

    Plasmid

    #133878

    Purpose

    5 copies of the NF-κβ DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    5 copies of the NF-kb DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. hHSF1 K80R

    Plasmid

    #118350

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to R.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-MBP-2xNLS-tdTomato

    Plasmid

    #104054

    Purpose

    An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-tdTomato
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    MBP

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MAC-NRP1

    Plasmid

    #158384

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    NRP1 (NRP1 Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pBAD-iLACCO1

    Plasmid

    #218817

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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