We narrowed to 11,215 results for: Kars

1. pJT25_GalL_BE3

   Plasmid

   #143736

   Purpose

   Expresses BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a_SIX1_P2A_Hygro_Barcode

   Plasmid

   #120476

   Purpose

   Barcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SIX1 (SIX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSMP-SUZ12_1

   Plasmid

   #36390

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   SUZ12 (SUZ12 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. VVT1

   Plasmid

   #65779

   Purpose

   The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SaCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. DHFR-GFP-th-GluA1

   Plasmid

   #173008

   Purpose

   For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   DHFR-GFP-th-GluA1
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHS1477

   Plasmid

   #205970

   Purpose

   MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas5-HNH all proteins, with His-tagged Cas11
   

   Use

   Tags

   14xHis on Cas11

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSMP-Dot1L_2

   Plasmid

   #36340

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   Dot1L (DOT1L Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pFE-hCA

   Plasmid

   #132999

   Purpose

   pFE carrying a human carbonic anhydrase II gene (Uniprot: P00918)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Insert

   hCAII (CA2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetR

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. DCT.VV.Orf106

   Plasmid

   #12794

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf106
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

    Plasmid

    #167268

    Purpose

    Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HOB
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSMP-EZH1_2

    Plasmid

    #36360

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EZH1 (EZH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-mCherry-LKB1(KD)

    Plasmid

    #84640

    Purpose

    mCherry-tagged, LKB1(K78I); kinase-dead mutant

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(K78I) (STK11 Synthetic, Human)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Lysine 78 mutated to Isoleucine; kinase dead

    Promoter

    CMV

    Available since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TPI-PTC1

    Plasmid

    #130699

    Purpose

    Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid)

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-PTC1 (TPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PTC1

    Promoter

    Pcmv

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HP21-TPI-WT-no_introns

    Plasmid

    #131044

    Purpose

    Expresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTR

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-Wildtype-no introns (TPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1

    Plasmid

    #176537

    Purpose

    Non-protein coding locus components from KraIscB-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    non-coding locus components from KraIscB-1 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTH727-CEN-RLuc/staCFLuc

    Plasmid

    #38211

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. phFluc-WT

    Plasmid

    #177714

    Purpose

    Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

    Depositor

    Erik Böttger

    Article

    Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

    Insert

    hFluc WT
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSMP-EZH1_3

    Plasmid

    #36361

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EZH1 (EZH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

19. p53-(1-393)-R273H-N268D

    Plasmid

    #35087

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1-393 - R273H-N268D

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCAGGS-Flag-hsDicer (Y971A/Y972A)

    Plasmid

    #41590

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y971A/Y972A

    Promoter

    CMV

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only