We narrowed to 7,663 results for: ski

1. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

   Plasmid

   #193915

   Purpose

   Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Farnesylation signal sequence and Flag tag

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX Slap2-SH2

   Plasmid

   #46501

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Slap2 (SLA2 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 84-197

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX Lnk-SH2

   Plasmid

   #46451

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Lnk (Sh2b3 Mouse)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 325-466

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNAS1b split mCherry NEDD4-2 WW2

   Plasmid

   #111883

   Purpose

   Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Type

   Empty backbone

   Tags

   Split mCherry system: NEDD4-2 WW2 fused to C-term…

   Expression

   Bacterial

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SIN40C.SFFV.mTagBFP2.let-7c

   Plasmid

   #169310

   Purpose

   Constitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   let-7c (MIRLET7C Human)
   

   Use

   Lentiviral

   Tags

   TagBFP2

   Promoter

   SFFV

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SIN40C.SFFV.dTomato.miR-125b-2

   Plasmid

   #169302

   Purpose

   Constitutive overexpression of miR-125b-2 with dTomato as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   miR-125b-2 (MIR125B2 Human)
   

   Use

   Lentiviral

   Tags

   dTomato

   Promoter

   SFFV

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDB181

   Plasmid

   #80692

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Insert

   gEHEE_06
   

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSECB sgMTHFD2L_1

   Plasmid

   #106306

   Purpose

   Express Cas9 and sgRNA targeting MTHFD2L

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD2L (MTHFD2L Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSECB sgMTHFD2L_2

   Plasmid

   #106307

   Purpose

   Express Cas9 and sgRNA targeting MTHFD2L

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD2L (MTHFD2L Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX p85b(NC)-SH2

    Plasmid

    #46468

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p85b(NC) (PIK3R2 Bovine)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 311-717

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX Nck2-SH2

    Plasmid

    #46458

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    NM_001004720.2 (NCK2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 274-380

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX Tem6-SH2

    Plasmid

    #46523

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Tem6 (TNS3 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1171-1291

    Promoter

    Tac

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPHR-EYFP

    Plasmid

    #103817

    Purpose

    Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-EYFP (CRY2 Mustard Weed)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX Vrap-SH2

    Plasmid

    #46531

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Vrap (SH2D2A Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 93-192

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBabe_puro_DEST_Flag_TLOC1 (220aa)

    Plasmid

    #45273

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCas9GG

    Plasmid

    #131009

    Purpose

    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Bba_R0040 TetR

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX BRDG1-SH2

    Plasmid

    #46410

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    BRDG1 (STAP1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 167-283

    Promoter

    Tac

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 HRPT2 L64P

    Plasmid

    #11052

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 L64P (CDC73 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L64P

    Available Since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pGEX Shb-SH2

    Plasmid

    #46485

    Depositor

    Bruce Mayer, Michael Welsh

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Shb (SHB Human)
    

    Tags

    GST, HA, and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 401-508

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5 Myc Rheb Omp25

    Plasmid

    #112764

    Purpose

    express myc-Rheb-Omp25

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    myc-Rheb-Omp25 (RHEB Human)
    

    Tags

    Omp25 and myc

    Expression

    Mammalian

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge