We narrowed to 8,227 results for: sars

1. pCMV-dLight1.2

   Plasmid

   #111054

   Purpose

   Expresses dLight1.2 in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.2
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. LacI-pLacI-pII pm

   Plasmid

   #218099

   Purpose

   Phagemid that expresses LacI, and pII under the Lac promoter

   Depositor

   Kevin Esvelt

   Insert

   pII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

   Plasmid

   #174890

   Purpose

   Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   U25C PylT

   Promoter

   EF1

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD2148

   Plasmid

   #153968

   Purpose

   pCMV6-Entry vector encoding human IRAK1 protein 196F

   Depositor

   Dirk Dittmer

   Article

   Seltzer et al J Virol. 2020 May 4;94(10). pii: JVI.021

   Insert

   IRAK1 (IRAK1 Human)
   

   Use

   Tags

   Myc-DDK

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TetO-FUW-Rfx4

   Plasmid

   #61544

   Purpose

   Expresses Rfx4 in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   Rfx4 (Rfx4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA flag PPAR gamma E499Q

   Plasmid

   #8896

   Depositor

   Bruce Spiegelman

   Article

   Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

   Insert

   PPAR gamma (Pparg Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   E499Q, a mutant in the AF2 domain. Binds PPARg l…

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDule2-Acd82

   Plasmid

   #174100

   Purpose

   Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

   Depositor

   Ryan Mehl

   Article

   Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

   Insert

   Acridone 82 tRNA synthetase and tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N311S, C313G, V366A, W382T

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA_RGG-GFP-RGG_withTEVsite

   Plasmid

   #124939

   Purpose

   Expresion of RGG_GFP-RGG

   Depositor

   Matthew Good, Benjamin Schuster, Daniel Hammer

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   TEV site between RGG and EGFP

   Promoter

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. NPY-pHTomato

   Plasmid

   #83501

   Purpose

   Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

   Depositor

   Sebastian Barg

   Article

   Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

   Insert

   NPY (NPY Human)
   

   Use

   Tags

   pHTomato

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTE4396

    Plasmid

    #74041

    Purpose

    Expresses human codon-optimized AsCpf1 and As crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    As crRNA
    AsCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pF420

    Plasmid

    #123659

    Purpose

    Produces cofactor F420 in E. coli

    Depositor

    Colin Jackson

    Article

    Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

    Inserts

    CofD
    FbiB
    FbiD
    FbiC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_R0040

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-HA-C12orf66

    Plasmid

    #87048

    Purpose

    mammalian expression of C12orf66

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    C12orf66 (C12orf66 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. bArr2-V5

    Plasmid

    #201494

    Purpose

    Expression of the beta-arrestin2 containing a N-terminal V5 tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    bArr2-V5 (ARRB2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVRa41_491

    Plasmid

    #49702

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF41_491
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVRa42_4454

    Plasmid

    #49703

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF42_4454
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiCRISPRv1 sgTollip

    Plasmid

    #196546

    Purpose

    Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

    Plasmid

    #198337

    Purpose

    PB plasmid expressing Halo-tagged PP7 coat protein

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    PP7 coat protein 3x Halo-GB1
    

    Use

    Piggybac

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. LacI-pLacI-pIV pm

    Plasmid

    #218101

    Purpose

    Phagemid that expresses LacI, and pIV under the Lac promoter

    Depositor

    Kevin Esvelt

    Insert

    pIV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-HA-KPTN

    Plasmid

    #87042

    Purpose

    mammalian expression of KPTN

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    KPTN (KPTN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMP71-super-IL-2

    Plasmid

    #174599

    Purpose

    Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAT9651-BEAR-GFP

    Plasmid

    #162989

    Purpose

    BEAR target plasmid with split EGFP and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPB12A

    Plasmid

    #210035

    Purpose

    Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas12a
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pyc111s

    Plasmid

    #158781

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Zachary Knight, Jennifer Garrison

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6s-RPL10a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK5-HA-ITFG2

    Plasmid

    #87045

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR

    Plasmid

    #67931

    Purpose

    Translational reporter - ARHGAP5 UTRs

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    sfGFP-NLS-P2A-NLS-mCherry (ARHGAP5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMSCV mSin1.2 myc

    Plasmid

    #12577

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Aug 15. ():.

    Insert

    mSin1.2 (MAPKAP1 Human)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMSCV mSin1.5 myc

    Plasmid

    #12579

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Aug 15. ():.

    Insert

    mSin1.5 (MAPKAP1 Human)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

29. pTACR TH1-p2aGFP

    Plasmid

    #135815

    Purpose

    CRISPR based on px330 p2aGFP targeting TH gene near stop codon

    Depositor

    Tim Ahfeldt, Lee Rubin

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH 1 guide
    

    Use

    CRISPR

    Tags

    p2a Myr-eGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTACR TH2-p2aGFP

    Plasmid

    #135816

    Purpose

    CRISPR based on px330 p2aGFP targeting TH gene near stop codon

    Depositor

    Tim Ahfeldt, Lee Rubin

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH 2 guide
    

    Use

    CRISPR

    Tags

    p2a Myr-eGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge