We narrowed to 18,161 results for: Met;

1. ES-FUCCI

   Plasmid

   #62451

   Purpose

   SMX_DEST vector with 3 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
   minPGK::Cherry-hCdt1(30-120)-bGHpA
   PGK::HygroR-bGpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB533_15F11-sfGFP

   Plasmid

   #167526

   Purpose

   H4K20me1-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Sato et al J Mol Biol. 2016 Oct 9;428(20):3885-3902

   Insert

   15F11-scFv
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPY22-mNeonGreen-Hyg

   Plasmid

   #137082

   Purpose

   Codon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patens

   Depositor

   Gohta Goshima

   Article

   Yi et al Curr Biol. 2020 Jul 20;30(14):2860-2868.

   Insert

   mNeonGreen-Flag
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCm C-term MBP NikJC199U

   Plasmid

   #174365

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Use

   Tags

   8xHis, MBP, and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-HA-SAMTOR

   Plasmid

   #100513

   Purpose

   Expresses HA tagged SAMTOR in mammalian cells

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   SAMTOR (BMT2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1-FLAG-Ku80

   Plasmid

   #46958

   Purpose

   Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku80 (XRCC5 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAL-C2X dAmp/Kana+

   Plasmid

   #154352

   Purpose

   Empty backbone vector

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLHDR03_mCherry_tCD19_unique guide

   Plasmid

   #124062

   Purpose

   Construction of 1st donor for scarless genome editing with unique guide

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Inserts

   mCherry
   tCD19
   

   Use

   Mouse Targeting; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Human UbC modified

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK003

   Plasmid

   #236570

   Purpose

   Cloning vector for PaqCI Golden Gate assembly of ISDra2 guides into ISDra2 plant expression vector

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEX-A-U6-gRNA

    Plasmid

    #65626

    Purpose

    Empty vector for the expression U6 driven gRNA

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    U6-gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUPD2+p35SoTMV

    Plasmid

    #170876

    Purpose

    Phytobrick - p35SoTMV

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    p35SoTMV
    

    Use

    Tags

    Expression

    Plant

    Mutation

    214C>T and 229C>T to remove BbsI restrictio…

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPAmCherry2-C1

    Plasmid

    #31932

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/K69N/L84F/A147T/S148L/I165L/Q167A/L169T/A179…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDN-Sth1Cas9

    Plasmid

    #221386

    Purpose

    Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

    Depositor

    Deborah Hung

    Article

    Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

    Insert

    Sth1 Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDN-Cherry-sgRNA

    Plasmid

    #221387

    Purpose

    episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

    Depositor

    Deborah Hung

    Article

    Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

    Insert

    none, cloning vector for introduction of targeting sequences
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICE-HA-NLS-I-PpoI

    Plasmid

    #46963

    Purpose

    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    NLS-I-PpoI
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TET

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. IMS-APEX2

    Plasmid

    #79058

    Purpose

    Expresses APEX in the mitochondrial intermembrane space.

    Depositor

    Alice Ting

    Article

    Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

    Insert

    APEX
    

    Use

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-C1-FLAG-Ku70

    Plasmid

    #46957

    Purpose

    Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    Ku70 (XRCC6 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRU294

    Plasmid

    #167689

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. G-CaMP3

    Plasmid

    #22692

    Purpose

    a single-wavelength GCaMP2-based genetically encoded calcium indicator

    Depositor

    Loren Looger

    Article

    Tian et al Nat Methods. 2009 Dec;6(12):875-81.

    Insert

    G-CaMP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N363D mutation (increased fluorescence)

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. #2.4 pre-G-SynapShot-Myc

    Plasmid

    #214722

    Purpose

    Expresses green #2.4 pre-SynapShot and Myc tag

    Depositor

    Won Do Heo

    Article

    Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

    Insert

    #2.4 pre-G-SynapShot
    

    Use

    AAV

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HA-mios in pRK5

    Plasmid

    #154212

    Purpose

    N-terminal HA-tagged Mios cDNA expression vector for transfections

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    Mios
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    cDNA sequence has been codon optimized using huma…

    Promoter

    CMV

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmiRFP680-N1

    Plasmid

    #136557

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP680
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMP11

    Plasmid

    #215559

    Purpose

    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Inserts

    Cas9
    gRNA targeting pBR322 origin of replication
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105 and tet

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX459_gRNA pAS dual_hspCas9

    Plasmid

    #193312

    Purpose

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCX-mutSpiCee

    Plasmid

    #140903

    Purpose

    mammalian expression of a mutated form of SpiCee, that does not affect calcium signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    mutSpiCee
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMSP1E3D1_D73C

    Plasmid

    #39328

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009 . 464():211-31.

    Insert

    MSP1E3D1_D73C (APOA1 synthetic gene)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    cys mutant of MSP1E3D1

    Promoter

    T7

    Available since

    Sept. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

27. Fuw-dCas9-Dnmt3a_IM

    Plasmid

    #84478

    Purpose

    Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a_IM
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pETM6-At4CL-CmCHS-MsCHI

    Plasmid

    #73394

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    MsCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

    Plasmid

    #121944

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGWB508-cTP-sfGFP

    Plasmid

    #213863

    Purpose

    Binary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    Plastid transit peptide fused sfGFP
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSF280

    Plasmid

    #167675

    Purpose

    Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGWB508-cTP-mCherry

    Plasmid

    #213862

    Purpose

    Binary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    Plastid transit peptide fused mCherry-UnaG
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CMV-mito-Citroff1-S245

    Plasmid

    #134306

    Purpose

    mitochondria-targeted inverse-response citrate sensor variant with medium binding affinity

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citroff1 S245A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBroad-T7RNAP-apraR

    Plasmid

    #199993

    Purpose

    T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    T7RNAP Circuit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    copFnull

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBABE-puro HGF

    Plasmid

    #10901

    Depositor

    Bob Weinberg

    Article

    Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

    Insert

    HGF (HGF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRK5-HA-YFP-raptor

    Plasmid

    #73385

    Purpose

    Expresses YFP and HA tagged Human raptor

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    Regulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

37. 2xMyc-tdMCP-SNAP (tdMCP standard)

    Plasmid

    #226291

    Purpose

    Lentiviral plasmid expressing a tdMCP-protein standard. Used in LABEL-seq abundance assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Inserts

    2xMyc-tdMCP-SNAPtag
    EGFP-IRES-BlastR
    

    Use

    Lentiviral

    Tags

    2xMyc-tdMCP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pdCas9-Tet1-CD

    Plasmid

    #83340

    Purpose

    To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

    Depositor

    Ronggui Hu

    Article

    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

    Insert

    dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    U6, CBH

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MCP/PCP RNA Lantern

    Plasmid

    #226095

    Purpose

    RNA tracking

    Depositor

    Jennifer Prescher

    Article

    Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

    Insert

    NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti_CMV:Flag-NLuc_SV40:PuroR

    Plasmid

    #183045

    Purpose

    Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    NanoLuciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge