Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 17,912 results for: REV

Showing: 10301 - 10350 of 17912 results

  5. pAV10

    Plasmid
    #63213
    Purpose
    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    63213-plasmid-map-sequence-id-113915

    Enlarge

  23. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

    Enlarge

  30. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Tight TRE promoter
    Available since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

    Enlarge

  32. pAF112

    Plasmid
    #135920
    Purpose
    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Type
    Empty backbone
    Use
    Luciferase; Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135920-plasmid-map-sequence-id-270078

    Enlarge

  34. lenti dCAS-VP64_Blast

    Plasmid
    #61425
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Lentiviral Prep
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    61425-plasmid-map-sequence-id-457486

    Enlarge

  37. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    103865-plasmid-map-sequence-id-243540

    Enlarge

  38. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid
    #138150
    Purpose
    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    hU6_Rfxcas13d-DR1_BsmBI
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    DR1 A>T
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138150-plasmid-map-sequence-id-350242

    Enlarge

  41. pLV-EGFP-Cre

    Plasmid
    #86805
    Purpose
    Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter
    Depositor
    Amy Lasek
    Article
    Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02
    Insert
    Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP and nuclear localization signal: PKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    86805-plasmid-map-sequence-id-436570

    Enlarge

  46. pLentiGuide

    Plasmid
    #117986
    Purpose
    pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.
    Depositor
    Paul Khavari
    Article
    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31
    Insert
    sgRNA backbone (F+E)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    117986-plasmid-map-sequence-id-235371

    Enlarge

  49. lenti_dCas9-KRAB-MeCP2

    Plasmid
    #122205
    Purpose
    Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.
    Depositor
    Andrea Califano
    Article
    Tan et al bioRxiv 2021.06.28.449297
    Insert
    dCas9-KRAB-MeCP2
    Use
    Lentiviral
    Tags
    KRAB-MeCP2
    Expression
    Mammalian
    Mutation
    Cas9 (D10A, H840A)
    Promoter
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122205-plasmid-map-sequence-id-377395

    Enlarge

Showing: 10301 - 10350 of 17912 results