We narrowed to 12,282 results for: LEA
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Plasmid#79516PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPANK2 (PANK2 Human)
UseBaculovirus expressionTags6xHis and TEV cleavage sitePromoterpolyhedrinAvailable sinceJune 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTriEx-mCherry-LINXa3-nano
Plasmid#73616PurposeLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLINXa3-nano
TagsmCherryExpressionBacterial, Insect, and Mamm…Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - CA
Plasmid#65424PurposeRhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63LDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, Q63L in RhoCAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - DN
Plasmid#65423PurposeRhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19NDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, T19N in RhoCAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F3shRNA human
Plasmid#66885PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F3DepositorAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F2shRNA human
Plasmid#66884PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F2DepositorAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre2_60.F2
Plasmid#60210PurposeExpresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)DepositorInsertCre recombinase (CD207 Synthetic, Human, Bacteriophage P1)
TagsFKBP12(F36V) and Nuclear localisation signalExpressionMammalianMutationFKBP12 has the F36V mutation; amino acids 60-343 …PromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-FAK30
Plasmid#59131PurposeCloning vector for FAK fret sensor with 30 nm helixDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFocal Adhesion Kinase
TagsFLAG, mCerulean, and mCitrineExpressionMammalianPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEMS1158
Plasmid#29073PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPle30 (CD68 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSAvailable sinceAug. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
TDRD3 (3PMT)
Plasmid#28274DepositorInsertTudor domain-containing protein 3 (TDRD3 Human)
TagsHis with TEV cleavageExpressionBacterialMutationcontains Tudor domain, residues 553-611.Available sinceApril 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
EC2_10_dCas9_VPR_HC_sgRNA
Plasmid#163712PurposeYeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC2_4_dCas9_VPR_sgRNA
Plasmid#163709PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-nCas9-UdgX
Plasmid#163566PurposeMammalian CG-to-GC base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUdgX-EE-UdgX-nCas9-UdgX
UseCRISPRExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)Available sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUG-T2A-Pten
Plasmid#140738PurposeLentiviral vector to overexpress human PTEN and GFPDepositorAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NLS
Plasmid#118938PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
ExpressionPlantAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-N-P-M-nucBFP-EGFP-L
Plasmid#52485PurposeExpresses nuclear mTagBFP and EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsnuclear mTagBFP
EGFP
UseRabies virusExpressionMammalianPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
cftr_R (OZ518)
Plasmid#27193DepositorInsertZinc finger array targeting cftr (cftr Zebrafish)
UseZebrafish targetingAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
ICBP90
Plasmid#25596PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-PM-InPAkt
Plasmid#181915PurposeIndicator of Phosphoinositides using Akt; targeted to the plasma membrane.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpm-InPAkt
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationContains the following mutations with respect to …PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C(RIT)-FLAG-AVITEV
Plasmid#74053Purposeretroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsAVI-TEV and FLAGExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only