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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36,291 results for: lat

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Showing: 4141 - 4160 of 36291 results

  4. pHRE

    Plasmid
    #134909
    Purpose
    Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.
    Depositor
    George Lomonossoff
    Publication
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    double 35S
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    134909-plasmid-map-sequence-id-267026

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  8. pTP434

    Plasmid
    #104167
    Purpose
    Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling
    Depositor
    Dirk Görlich
    Publication
    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554
    Insert
    Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
    Tags
    14x Histidine tag and NEDD8 from Brachypodium dis…
    Expression
    Bacterial
    Mutation
    3x Cysteines located at bps 493-495, 520-522, and…
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104167-plasmid-map-sequence-id-200061

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  18. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid
    #115694
    Purpose
    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer
    Publication
    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NmeCas9
    Nme-sgRNA
    Use
    CRISPR
    Tags
    NLS and NLS, HA
    Expression
    Insect and Mammalian
    Mutation
    None and fusion of repeat/tracrRNA to make a sgRNA
    Promoter
    EF-1 alpha and U6
    Available since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115694-plasmid-map-sequence-id-221986

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  19. CD8-GFP-FKBP

    Plasmid
    #100751
    Purpose
    Expression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.
    Depositor
    Stephen Royle
    Publication
    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD8A (CD8A Human)
    Tags
    FKBP and GFP
    Expression
    Mammalian
    Mutation
    Truncated
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    100751-plasmid-map-sequence-id-194049

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Showing: 4141 - 4160 of 36291 results