We narrowed to 9,957 results for: pET

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pET29a-hNABP1-N

Plasmid

#128310

Purpose

Express hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP1 N-terminal domain (OB fold, 1–141 aa) (NABP1 Human)

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (OB fold, 1–140 aa)

Promoter

T7

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2-C

Plasmid

#128309

Purpose

Express hNABP2 C-terminus(122–211 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 C-terminal domain (122–211 aa) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

C-terminal domain (122–211 aa)

Promoter

T7

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-INTS3-C

Plasmid

#128642

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 C-termini (INTS3 Human)

Tags

His tag on N and C-terminal

Expression

Bacterial

Mutation

INTS3 C-termini (514–1042 aa)

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3/S3

Plasmid

#124627

Purpose

Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Publication

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 237 to 241 replaced with corresponding s…

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Publication

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Publication

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-MetN

Plasmid

#114458

Purpose

Expression of hexahistidine-tagged AceS methylase in E. coli

Depositor

Patrick Caffrey

Publication

Sheehan et al Mol Biosyst. 2017 May 2;13(5):866-873. d

Insert

aceS

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFS_0376_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1_RC

Plasmid

#116988

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0357_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1

Plasmid

#116969

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0358_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1_RC

Plasmid

#116970

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0369_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1

Plasmid

#116981

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0380_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3_RC

Plasmid

#116992

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0359_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_2

Plasmid

#116971

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-YJR129c

Plasmid

#85118

Purpose

bacterial expression of YJR129c

Depositor

Pal Falnes

Publication

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFM3 (EFM3 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0366_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_2_RC

Plasmid

#116978

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0367_pET30-RT-CRISPR_Woodsholea_maritima_ARRAY_1

Plasmid

#116979

Purpose

Expresses WmRT-Cas1-Cas2 under pT7lac promoter, encodes WmCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Woodsholea maritima RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0368_pET30-RT-CRISPR_Woodsholea_maritima_ARRAY_1_RC

Plasmid

#116980

Purpose

Expresses WmRT-Cas1-Cas2 under pT7lac promoter, encodes WmCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Woodsholea maritima RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0361_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_3

Plasmid

#116973

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 3, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0363_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_1

Plasmid

#116975

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0379_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3

Plasmid

#116991

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge