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We narrowed to 2,781 results for: PREP

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Showing: 601 - 630 of 2781 results

  1. pSL103-SpCas9-FLAG-3XNLS

    Plasmid
    #182032
    Purpose
    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP
    Depositor
    Steven Lin
    Article
    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do
    Insert
    Streptococcus pyogenes cas9 (cas9 Synthetic)
    Use
    CRISPR
    Tags
    12X His tag, 3XNLS, FLAG, and MBP
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    182032-plasmid-map-sequence-id-360101

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  10. pMSCV-BLU-VIPR

    Plasmid
    #220497
    Purpose
    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Expression
    Mammalian
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220497-plasmid-map-sequence-id-442817

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  19. pSBL_DK186

    Plasmid
    #156422
    Purpose
    Mammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein
    Tags
    EGFP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156422-plasmid-map-sequence-id-306308

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  22. pSBL_DK198

    Plasmid
    #156425
    Purpose
    Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Tags
    sfGFP-His
    Expression
    Insect
    Mutation
    aa 42-275
    Promoter
    Polyhedrin
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    156425-plasmid-map-sequence-id-307748

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  23. pNB-Rep

    Plasmid
    #238996
    Purpose
    Node B carrying the sfGPF reporter that can be used for the assembly of pEND-11Y, pEND-Y11, pEND-Z11 or pEND-ZY
    Depositor
    Javier Santos-Moreno
    Article
    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2
    Insert
    sfGFP-MarAn10 downstream of a strong constitutive promoter and a binding site for sgRNA-1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    238996-plasmid-map-sequence-id-495027

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  24. pBA1751

    Plasmid
    #246066
    Purpose
    Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pJex promoter; SC101 ori
    Depositor
    Jennifer Doudna
    Article
    Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2
    Insert
    Panoptes locus
    Expression
    Bacterial
    Promoter
    pJEX promoter
    Available Since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    246066-plasmid-map-sequence-id-491110

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  27. pLVX-TetOne-bleo-atpD-K155Q

    Plasmid
    #248068
    Purpose
    Lentiviral expression of a catalytically inactive mutant of the beta subunit of ATPGobble. Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-puro-atpG (#248067) for a LOF control.
    Depositor
    Denis Titov
    Article
    Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.
    Insert
    atpD
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    Lysine 155 to Glutamine, codon-optimized for expr…
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    248068-plasmid-map-sequence-id-493802

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  28. pLVX-TetOne-puro-atpG

    Plasmid
    #248067
    Purpose
    Lentiviral expression of gamma subunit of ATPGobble. Includes ampR and puroR (puromycin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-bleo-atpD (#248066) for active ATPGobble.
    Depositor
    Denis Titov
    Article
    Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.
    Insert
    atpG (atpG E. coli)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    codon-optimized for expression in human cells
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    248067-plasmid-map-sequence-id-493799

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  29. pLVX-TetOne-bsd-atpA

    Plasmid
    #248065
    Purpose
    Lentiviral expression of alpha subunit of ATPGobble. Includes ampR and bsdR (blasticidin). Use together with pLVX-TetOne-bsd-atpD (#248066) and pLVX-TetOne-atpG (#248067) for active ATPGobble.
    Depositor
    Denis Titov
    Article
    Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.
    Insert
    atpA
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in human cells
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    248065-plasmid-map-sequence-id-493789

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Showing: 601 - 630 of 2781 results